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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

IM33 Sigma-AldrichAnti-MMP-2 (Ab-3) Mouse mAb (42-5D11)

This Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-2 (Ab-3).

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

同义词: Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, H, M, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~72 kDa latent and the ~66 kDa active forms of the MMP-2.
Catalogue Number	IM33
Brand Family	Calbiochem®
Synonyms	Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application Data	Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) (Cat. No. IM33) (2 µg/ml). Detection: chemiluminescence.

References
References	Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F. 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

Product Information
Declaration	Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Form	Liquid
Formulation	In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Negative control	MMP-9 protein (Cat. Nos. PF024 or PF038)
Positive control	MMP-2 protein (Cat. Nos. PF023 or PF037)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. Immunoblotting, Original Clone Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsuchiya, Y., et al. 1994. Int. J. Cancer 56, 46.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Paraffin Sections
Application Notes	Frozen Sections (see application references) Immunoblotting (1 µg/ml) Paraffin Sections (see application references)
Application Comments	This antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2
Immunogen	Human
Epitope	within amino acids 468-483 of human MMP-2 (numbered from the propeptide)
Clone	42-5D11
Host	Mouse
Isotype	IgG₁
Species Reactivity	Bovine Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
IM33-100UGCN	07790788053772

Documentation

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) MSDS

职位
物料安全数据表 (MSDS)

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) 分析证书

标题	批号
IM33	输入批号

参考

参考信息概述
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F. 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

小册子

标题
Angiogenesis and Tumor Metastasis Brochure and Technical Guide

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	20-July-2007 JSW
Synonyms	Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application	Frozen Sections (see application references) Immunoblotting (1 µg/ml) Paraffin Sections (see application references)
Application Data	Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts NB1RGB cells. Primary antibody: Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) (Cat. No. IM33) (2 µg/ml). Detection: chemiluminescence.
Description	Purified mouse monoclonal antibody (see application references). Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
Background	Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin, and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus, and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-2 (Gelatinase A, 72 kDa gelatinase/type IV collagenase, TBE-1) is secreted as a 72 kDa zymogen which is proteolytically processed to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin, and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential, and suggest that it may be a useful marker for the diagnosis or prognosis of cancer.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2
Epitope	within amino acids 468-483 of human MMP-2 (numbered from the propeptide)
Clone	42-5D11
Isotype	IgG₁
Species	bovine, human, mouse, rat
Positive control	MMP-2 protein (Cat. Nos. PF023 or PF037)
Negative control	MMP-9 protein (Cat. Nos. PF024 or PF038)
Form	Liquid
Formulation	In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	This antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F. 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.
Application references	Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. Immunoblotting, Original Clone Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91. Paraffin Sections Tsuchiya, Y., et al. 1994. Int. J. Cancer 56, 46.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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