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MABS1250 Sigma-AldrichAnti-Mineralocorticoid Receptor Antibody, clone 2D6

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Nr3c2, Mlr UniProt Number: P22199

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB, IHC, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS1250
Description	Anti-Mineralocorticoid Receptor Antibody, clone 2D6
Alternate Names	MR Nuclear receptor subfamily 3 group C member 2 Mineralocorticoid Receptor
Background Information	Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Key Applications	Western Blotting Immunohistochemistry Immunocytochemistry
Application Notes	Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in hippocampus, dentate gyrate, choroid plexus, heart, and rat kidney (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348). Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671). Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in rat hippocampal cytosolic preparation and exogenously expressed EGFP-rat MR fusion protein in CHO cells (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348). Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney cytosolic fraction and exogenously expressed human MR in COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671)

Biological Information
Immunogen	Conjugated linear peptide corresponding to the N-terminus of rat Mineralocorticoid Receptor.
Epitope	Near N-terminus
Clone	2D6
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Epitope is present in all 3 alternative spliced isoforms
Isotype	IgG2aκ
Species Reactivity	Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_037263
Gene Symbol	Nr3c2 Mlr
Purification Method	Protein G Purified
UniProt Number	P22199
Molecular Weight	~107 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in rat brain cytosol tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of rat brain cytosol tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS1250	04055977173031

Documentation

Anti-Mineralocorticoid Receptor Antibody, clone 2D6 MSDS

职位
物料安全数据表 (MSDS)

Anti-Mineralocorticoid Receptor Antibody, clone 2D6 分析证书

标题	批号
Anti-Mineralocorticoid Receptor, -Q2573211	Q2573211
Anti-Mineralocorticoid Receptor, clone 2D6 - 3245604	3245604
Anti-Mineralocorticoid Receptor, clone 2D6 - 3884760	3884760
Anti-Mineralocorticoid Receptor, clone 2D6 - 4209927	4209927

技术信息

标题
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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