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AB5164-50UL Sigma-AldrichAnti-Muscarinic Acetylcholine Receptor m1 Antibody

Anti-Muscarinic Acetylcholine Receptor m1 Antibody is an antibody against Muscarinic Acetylcholine Receptor m1 for use in IH & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
数据表

Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRM1, M1, HM1, MGC30125 UniProt Number: P11229

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB5164-50UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Muscarinic Acetylcholine Receptor m1 Antibody

References

Product Information
Format	Affinity Purified
Control	CONTROL ANTIGEN: Included free of charge with the antibody is 60 μg of control antigen, molecular weight 40kDa (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality Level	MQ100

Applications
Application	Anti-Muscarinic Acetylcholine Receptor m1 Antibody is an antibody against Muscarinic Acetylcholine Receptor m1 for use in IH & WB.
Key Applications	Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:200 (1-1.5 μg/mL) using ECL on rat brain membranes with proteinase inhibitors. M1 is approximately 60-80kDa in size, some preparations yield 125kDa dimers (photo). Dilutions should be made using a carrier protein such as BSA (1-3%). Immunohistochemistry: rat brain frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	GST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes a full length m1 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
Species Reactivity	Human Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000738.2
Entrez Gene Summary	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
Gene Symbol	CHRM1 M1 HM1 MGC30125
UniProt Number	P11229
UniProt Summary	FUNCTION: SwissProt: P11229 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. SIZE: 460 amino acids; 51421 Da SUBUNIT: Interacts with GPRASP2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P11229 ## Belongs to the G-protein coupled receptor 1 family.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	50 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
AB5164-50UL	04053252506697

Documentation

Anti-Muscarinic Acetylcholine Receptor m1 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Muscarinic Acetylcholine Receptor m1 Antibody 分析证书

标题	批号
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR	2475607
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2424653	2424653
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR - 3510289	3510289
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR - 3597402	3597402
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2571233	2571233
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2689070	2689070
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2757183	2757183
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2772724	2772724
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2781861	2781861
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR -2788895	2788895

数据表

标题
RABBIT ANTI-M1 MUSCARINIC ACETYLCHOLINE RECEPTOR AFFINITY PURIFIED POLYCLONAL ANTIBODY

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产品目录编号

描述

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Anti-Muscarinic Acetylcholine Receptor m1 Antibody

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