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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

AP1160 Sigma-AldrichAnti-NDRG1 Mouse mAb (2D7)

This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.

Anti-NDRG1 Mouse mAb (2D7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
参考
Data Sheet

同义词: Anti-N-myc Downstream Regulator 1

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.
Catalogue Number	AP1160
Brand Family	Calbiochem®
Synonyms	Anti-N-myc Downstream Regulator 1
Application Data	Detection of human NDRG1 by immunoblotting. Sample: Full-length recombinant human NDRG1 protein. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (1-5 µg/ml). Detection: chemiluniescence. Detection of human NDRG1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (10 µg/ml). Detection: fluorescence. Detection of human NDRG1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human endometrium. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (0.7 µg/ml). Detection: colorimetric (DAB).

References
References	Jiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898. Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.

Product Information
Form	Liquid
Formulation	In PBS, pH 7.2.
Positive control	Human endometrium tissue, HeLa cells
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (0.7 µg/ml)
Application Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
Clone	2D7
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Regulatory Review
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
AP1160-100UGCN	04055977212662

Documentation

Anti-NDRG1 Mouse mAb (2D7) MSDS

职位
物料安全数据表 (MSDS)

参考

参考信息概述
Jiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898. Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-October-2010 RFH
Synonyms	Anti-N-myc Downstream Regulator 1
Application	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (0.7 µg/ml)
Application Data	Detection of human NDRG1 by immunoblotting. Sample: Full-length recombinant human NDRG1 protein. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (1-5 µg/ml). Detection: chemiluniescence. Detection of human NDRG1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (10 µg/ml). Detection: fluorescence. Detection of human NDRG1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human endometrium. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (0.7 µg/ml). Detection: colorimetric (DAB).
Description	Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.
Background	NDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.
Host	Mouse
Immunogen	Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
Clone	2D7
Isotype	IgG_2a
Species	human
Positive control	Human endometrium tissue, HeLa cells
Form	Liquid
Formulation	In PBS, pH 7.2.
Preservative	None
Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Regulatory Review
References	Jiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898. Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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