Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.5 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Clone
1E12
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-November-2010 RFH
Synonyms
Anti-Paired Box 7
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.5 µg/ml)
Application Data
Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence. Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
Background
This protein is a member of the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These proteins play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Clone
1E12
Isotype
IgG2a
Species
human, mouse
Positive control
NIH3T3 cells, Human stomach tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
