Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.8 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Clone
2G1
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-Pyruvate Dehydrogenase Kinase 2
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.8 µg/ml)
Application Data
Detection of human PDK2 by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (1-5 µg/ml). Detection: chemiluminescence. Detection of human PDK2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-PDK2 Mouse mAb (2G1) (Cat. No. ST1643) (0.8 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
Background
The PDK2 protein inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 α subunit, thus contributing to the regulation of glucose metabolism.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Clone
2G1
Isotype
IgG2a
Species
human
Positive control
U-2 OS cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
