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MABN1596 Sigma-AldrichAnti-Pan Shc Antibody, clone 3A12.1

Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.

Anti-Pan Shc Antibody, clone 3A12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: SHC1, SHC, SHCA UniProt Number: P29353

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN1596
Description	Anti-Pan Shc Antibody, clone 3A12.1
Alternate Names	p46Shc p52Shc p66Shc SHC-transforming protein 1 SH2 domain protein C1 SHC-transforming protein 3 SHC-transforming protein A Src homology 2 domain-containing-transforming protein C1
Background Information	SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.

Biological Information
Immunogen	GST-tagged recombinant human Shc C-terminal region fragment.
Epitope	C-terminal region.
Clone	3A12.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001123512
Gene Symbol	SHC1 SHC SHCA
Purification Method	Protein G purified.
UniProt Number	P29353
Molecular Weight	~47-63 kDa observed. 62.82 kDa (isoform p66Shc), 51.61 kDa (isoform p52Shc), 46.67 kDa (isoform p46Shc), 40.42 kDa (isoform 5), 62.89 kDa (isoform 6), 51.68 kDa (isoform 7) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN1596	04055977350944

Documentation

Anti-Pan Shc Antibody, clone 3A12.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-Pan Shc Antibody, clone 3A12.1 分析证书

标题	批号
Anti-Pan Shc, clone 3A12.1 - 3477005	3477005
Anti-Pan Shc, clone 3A12.1 - 3849550	3849550
Anti-Pan Shc, clone 3A12.1 - 3995081	3995081
Anti-Pan Shc, clone 3A12.1 - 4110910	4110910
Anti-Pan Shc, clone 3A12.1 -Q2683370	Q2683370

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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