Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant, human S100A7 (aa 1-102)
Clone
1A4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
29-October-2010 RFH
Synonyms
Anti-S100 Calcium Binding Protein A7
Application
ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Data
Detection of human S100A7 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: chemiluminescence. Detection of human S100A7 by immunocytochemistry. Sample: A-431 cells fixed in paraformaldehyde. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~10 kDa S100A7 protein.
Background
S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known.
Host
Mouse
Immunogen
Full-length, recombinant, human S100A7 (aa 1-102)
Clone
1A4
Isotype
IgG₁
Species
human
Positive control
A-431 cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
