Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.2 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
Immunogen
NA
Clone
6E3
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Cauffman, G., et al. 2009. Hum. Reprod.24 63. Forte, A., et al. 2009. Mol. Med.15 392.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
24-August-2015 JSW
Synonyms
Anti-SAL-Like 4
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (0.2 µg/ml)
Application Data
Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence. Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence. Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
Background
This protein may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).
Host
Mouse
Immunogen species
NA
Immunogen
A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
Clone
6E3
Isotype
IgG₁
Species
human, mouse
Positive control
HeLa cells, NIH3T3 cells, Human testes tissue
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Regulatory Review
References
Cauffman, G., et al. 2009. Hum. Reprod.24 63. Forte, A., et al. 2009. Mol. Med.15 392.