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Recognizes the ~18 kDa SKP1A protein in HeLa cells.
Catalogue Number
ST1709
Brand Family
Calbiochem®
Synonyms
Anti-S-Phase Kinase-Associated Protein 1
Application Data
Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence. Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence. Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~18 kDa SKP1A protein.
Background
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
Clone
1H8
Isotype
IgG2a
Species
human
Positive control
HeLa cells
Negative control
293T cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
