Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
Clone
4E3
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-October-2010 JLN
Synonyms
Anti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Receptor Like 2
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human TNFSF14 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (1-5 µg/ml). Detection: chemiluminescence. Detection of human TNFSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the TNFSF14 protein.
Background
TNFSF14 is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, a member of the tumor necrosis factor receptor superfamily, whereby TNFRSF14 is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
Clone
4E3
Isotype
IgG2a
Species
human
Positive control
Human spleen tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
