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612161
Sigma-AldrichAnti-Tissue Factor Mouse mAb (TF9-10H10)
This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor.
More>>This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor. Less<<
Anti-Tissue Factor Mouse mAb (TF9-10H10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Flow Cytometry
Veltrop, M.H., et al. 2001. Infect. Immun.69, 2797.
ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry
Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315.
Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies
Morrissey, J.H., et al. 1988. Thromb. Res.52, 247.
Immunoblotting
Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol.20, 874.
Key Applications
Dot Blot
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Not Neutralization Studies
Paraffin Sections
Application Notes
Dot Blot (see application references) ELISA (see application references) Flow Cytometry (see application references) Frozen Sections (see application references) Immunoblotting (1:1000, see application references) Immunofluorescence (see application references) Paraffin Sections (see application references) Neutralization Studies (not recommended)
Application Comments
Specific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified apoprotein of human tissue factor
Immunogen
Human
Epitope
within extracellular domain
Clone
TF9-10H10
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Primate
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
24-January-2016 JSW
Application
Dot Blot (see application references) ELISA (see application references) Flow Cytometry (see application references) Frozen Sections (see application references) Immunoblotting (1:1000, see application references) Immunofluorescence (see application references) Paraffin Sections (see application references) Neutralization Studies (not recommended)
Application Data
Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
Background
Tissue factor (TF) is the membrane receptor for coagulation factors VII and VIIa and is a cell-surface initiator of coagulation. It is the major molecule of this type and is critical for controlling hemostasis, thrombosis, and inflammation. TF is not generally present in or on cells within the vasculature, but following vascular injury, blood can come into contact with TF expressed by extravascular cells, resulting in activation of the coagulation cascade.
Host
Mouse
Immunogen species
Human
Immunogen
purified apoprotein of human tissue factor
Epitope
within extracellular domain
Clone
TF9-10H10
Isotype
IgG₁
Species
human, primate, not rabbit
Form
Lyophilized
Formulation
Lyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
Preservative
None
Comments
Specific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
Application references
Flow Cytometry
Veltrop, M.H., et al. 2001. Infect. Immun.69, 2797.
ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry
Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315.
Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies
Morrissey, J.H., et al. 1988. Thromb. Res.52, 247.
Immunoblotting
Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol.20, 874.
