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Recognizes the ~125 kDa ULK1 protein in human colon tissue.
Catalogue Number
ST1521
Brand Family
Calbiochem®
Synonyms
Anti-Unc-51-Like Kinase 1
Application Data
Detection of human ULK1 by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-ULK1 Mouse mAb (2H8) (Cat. No. ST1521) (1-5 µg/ml). Detection: chemiluminescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 602-716 of human ULK1, expressed as a GST fusion protein
Immunogen
NA
Clone
2H8
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-June-2010 RFH
Synonyms
Anti-Unc-51-Like Kinase 1
Application
ELISA (see coments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ULK1 by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-ULK1 Mouse mAb (2H8) (Cat. No. ST1521) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~125 kDa ULK1 protein.
Host
Mouse
Immunogen species
NA
Immunogen
A recombinant polypeptide corresponding to amino acids 602-716 of human ULK1, expressed as a GST fusion protein
Clone
2H8
Isotype
IgG₁
Species
human
Positive control
Human colon tissue
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
