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This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin.
More>>This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin. Less<<
Anti-Ubiquitin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
Catalogue Number
662099
Brand Family
Calbiochem®
Application Data
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
References
References
Jungmann, J., et al. 1993. Nature361, 369. Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem.61, 761. Hochstrasser, M. 1992. Curr. Opin. Cell Biol.4, 1024. Jentsch, S. 1992. Annu. Rev. Genet.26, 179. Hershko, A., et al. 1982. J. Biol. Chem.257, 13964.
Jungmann, J., et al. 1993. Nature361, 369. Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem.61, 761. Hochstrasser, M. 1992. Curr. Opin. Cell Biol.4, 1024. Jentsch, S. 1992. Annu. Rev. Genet.26, 179. Hershko, A., et al. 1982. J. Biol. Chem.257, 13964.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
Description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
Background
Ubiquitin is a highly conserved protein that functions in intracellular regulation. The ubiquitin system is the major pathway for selective protein degradation and is responsible for the degradation of abnormal and short-lived proteins. In the first step, ubiquitin is activated in an ATP-dependent reaction by ubiquitin-activating enzyme (E1). Activated ubiquitin is transferred to a ubiquitin conjugating enzyme (E2), then transferred to a protein substrate with the aid of ubiquitin-protein ligase (E3). An isopeptide linkage is formed between the C-terminus of the ubiquitin and an internal lysine residue of the substrate protein. Degradation requires the presence of a multi-ubiquitin chain on a substrate, which targets the protein for degradation by the 26S proteasome. Ubiquitin molecules are then released and recycled.
Host
Rabbit
Immunogen species
Bovine
Immunogen
bovine erytherocyte ubiquitin conjugated to KLH
Isotype
IgG
Species
Drosophila, E. coli, bovine, canine, chicken, frog, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep, yeast
Form
Liquid
Preservative
≤0.1% sodium azide
Comments
Variables associated with assay conditions will dictate the optimal working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C)
Toxicity
Standard Handling
References
Jungmann, J., et al. 1993. Nature361, 369. Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem.61, 761. Hochstrasser, M. 1992. Curr. Opin. Cell Biol.4, 1024. Jentsch, S. 1992. Annu. Rev. Genet.26, 179. Hershko, A., et al. 1982. J. Biol. Chem.257, 13964.
