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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP140 Sigma-AldrichAnti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7)

This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, H, Mk	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
Catalogue Number	OP140
Brand Family	Calbiochem®
Application Data	Detection of human p53 by staining paraffin sections. Sample: Human colon carcinoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml) Detection: DAB. Detection of human p53 by immunoblotting. Sample: Human p53. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml). Detection: chemiluminescence.

References
References	Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358,* 15.*

Product Information
Form	Liquid
Formulation	In 10 mM PBS, pH 7.4.
Positive control	SKBR-3 or MDA-231 cells or colon carcinoma tissue
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (1-2 µg/ml) Immunoblotting (1-2 µg/ml) Immunofluorescence (0.5 µg/ml) Immunoprecipitation (2 µg/mg lysate) Paraffin Sections (1-2 µg/ml)
Application Comments	Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant, human p53 expressed in E. coli
Immunogen	Human
Epitope	within amino acids 37-45 of p53
Clone	DO-7
Host	Mouse
Isotype	IgG_2b
Species Reactivity	Bovine Human Monkey
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OP140-100UGCN	07790788053871

Documentation

Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) MSDS

职位
物料安全数据表 (MSDS)

Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) 分析证书

标题	批号
OP140	输入批号

参考

参考信息概述
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358,* 15.*

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-March-2009 JSW
Application	Frozen Sections (1-2 µg/ml) Immunoblotting (1-2 µg/ml) Immunofluorescence (0.5 µg/ml) Immunoprecipitation (2 µg/mg lysate) Paraffin Sections (1-2 µg/ml)
Application Data	Detection of human p53 by staining paraffin sections. Sample: Human colon carcinoma fixed in formalin and paraffin-embedded. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml) Detection: DAB. Detection of human p53 by immunoblotting. Sample: Human p53. Primary antibody: Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) (Cat. No. OP140) (1 µg/ml). Detection: chemiluminescence.
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Background	The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells. Cat. No. OP140 recognizes a 53 kDa phosphoprotein. The antibody binds to both mutant and wild-type p53.
Host	Mouse
Immunogen species	Human
Immunogen	recombinant, human p53 expressed in E. coli
Epitope	within amino acids 37-45 of p53
Clone	DO-7
Isotype	IgG_2b
Species	bovine, human, monkey
Positive control	SKBR-3 or MDA-231 cells or colon carcinoma tissue
Form	Liquid
Formulation	In 10 mM PBS, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Lane, D.P. 1992. Nature 358,* 15.*

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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