数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

HCKP1-11K-PX17 MilliporeMILLIPLEX® Human Immuno-Oncology Checkpoint Protein Premixed 17-plex Panel 1 - Immuno-Oncology Multiplex Assay

The Human Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 17 immune checkpoint proteins in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
Protocols

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Oncology Research Sub-Category: Immune Response, Inflammation, Tumor Markers

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HCKP1-11K-PX17

96-well plate 96 well plate

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概述

Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
BTLA CD27 CD28 CD40 CD80/B7-1 CD86/B7-2 CTLA-4 GITR GITRL HVEM ICOS LAG-3 PD-1 PD-L1 PD-L2 TIM-3 TLR-2	H	Mplex	Luminex xMAP

Description
Catalogue Number	HCKP1-11K-PX17
Trade Name	MILLIPLEX
Description	MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Premixed 17-plex Panel 1 - Immuno-Oncology Multiplex Assay
Overview	The MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 1 is a 17-plex kit to be used for the simultaneous quantification of all of the following analytes in serum, plasma and tissue culture samples: BTLA (B- and T-lymphocyte attenuator), CD27 (Cluster of differentiation 27), CD28 (Cluster of differentiation 28), CD40 (Cluster of differentiation 40), CD80/B7-1 (Cluster of differentiation 80/B7-1), CD86/B7-2 (Cluster of differentiation 86/B7-2), CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), GITRL (Ligand for receptor TNFRSF18/AITR/GITR), GITR (Glucocorticoid-induced TNFR-related protein), HVEM (Herpesvirus entry mediator), ICOS (Inducible T-cell costimulator), LAG-3 (Lymphocyte-activation gene 3), PD-L1 (Programmed death-ligand 1), PD-L2 (Programmed death-ligand 2), PD-1 (Programmed cell death protein 1), TIM-3 (T-cell immunoglobulin and mucin-domain containing-3), TLR-2 (Toll like receptor 2).
Background Information	Immune checkpoint proteins are regulators of immune activation and play a key role in maintaining immune homeostasis, preventing autoimmunity, and are involved in cancer immune escape. These immune system molecules demonstrate the ability to act both as agonists (co-stimulatory checkpoint molecules) or antagonists (co-inhibitory checkpoint molecules) of immune response. Immune checkpoint inhibitor drugs targeting inhibitory immune checkpoint proteins, such as CTLA-4 and PD-L1, have demonstrated efficacy in cancer immunotherapy by improving the antitumor immune response and saving lives in the treatment of many malignancies. Immune checkpoint proteins may express on immune cells and/or tumor cells as activating receptors or inhibitory receptors. Many immune checkpoint proteins are also expressed as soluble forms in circulation and in the tumor and tumor microenvironment and are putative biomarkers for immunotherapy in various diseases including cancer.

References

Product Information
Detection method	Luminex xMAP
HS Code	3822 19 30
Configuration	Premixed
Precision, %	Inter-assay: <15 Intra-assay: <5
Panel Type	MAGNETIC Circulating Cancer

Applications
Application	The Human Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 17 immune checkpoint proteins in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	For optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommended) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human
Analytes Available	BTLA CD27 CD28 CD40 CD80/B7-1 CD86/B7-2 CTLA-4 GITR GITRL HVEM ICOS LAG-3 PD-1 PD-L1 PD-L2 TIM-3 TLR-2
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	BTLA: 95% CD27: 96% CD28: 95% CD40: 97% CD80/B7-1: 96% CD86/B7-2: 98% CTLA-4: 95% GITR: 90% GITRL: 103% HVEM: 98% ICOS: 100% LAG-3: 97% PD-1: 97% PD-L1: 97% PD-L2: 102% TIM-3: 98% TLR-2: 95%

Physicochemical Information
Sensitivity	(minDC+ 2SD) BTLA: 43.8 pg/mL CD27: 24.1 pg/mL CD28: 84.5 pg/mL CD40: 4.3 pg/mL CD80/B7-1: 11.2 pg/mL CD86/B7-2: 86.1 pg/mL CTLA-4: 9.3 pg/mL GITR: 18.8 pg/mL GITRL: 20.5 pg/mL HVEM: 0.8 pg/mL ICOS: 55.6 pg/mL LAG-3: 66.0 pg/mL PD-1: 13.7 pg/mL PD-L1: 1.3 pg/mL PD-L2: 46.6 pg/mL TIM-3: 1.5 pg/mL TLR-2: 24.1 pg/mL
Standard Curve Range	BTLA: 49 – 200,000 pg/mL CD27: 24 – 100,000 pg/mL CD28: 244 – 1,000,000 pg/mL CD40: 5 – 20,000 pg/mL CD80/B7-1: 12 – 50,000 pg/mL CD86/B7-2: 73 – 300,000 pg/mL CTLA-4: 12 – 50,000 pg/mL GITR: 5 – 20,000 pg/mL GITRL: 24 – 100,000 pg/mL HVEM: 2 – 10,000 pg/mL ICOS: 49 – 200,000 pg/mL LAG-3: 122 – 500,000 pg/mL PD-1: 24 – 100,000 pg/mL PD-L1: 5 – 20,000 pg/mL PD-L2: 49 – 200,000 pg/mL TIM-3: 5 – 20,000 pg/mL TLR-2: 73 – 300,000 pg/mL

Physicochemical Information

Sensitivity

(minDC+ 2SD)
BTLA: 43.8 pg/mL
CD27: 24.1 pg/mL
CD28: 84.5 pg/mL
CD40: 4.3 pg/mL
CD80/B7-1: 11.2 pg/mL
CD86/B7-2: 86.1 pg/mL
CTLA-4: 9.3 pg/mL
GITR: 18.8 pg/mL
GITRL: 20.5 pg/mL
HVEM: 0.8 pg/mL
ICOS: 55.6 pg/mL
LAG-3: 66.0 pg/mL
PD-1: 13.7 pg/mL
PD-L1: 1.3 pg/mL
PD-L2: 46.6 pg/mL
TIM-3: 1.5 pg/mL
TLR-2: 24.1 pg/mL

Standard Curve Range

BTLA: 49 – 200,000 pg/mL
CD27: 24 – 100,000 pg/mL
CD28: 244 – 1,000,000 pg/mL
CD40: 5 – 20,000 pg/mL
CD80/B7-1: 12 – 50,000 pg/mL
CD86/B7-2: 73 – 300,000 pg/mL
CTLA-4: 12 – 50,000 pg/mL
GITR: 5 – 20,000 pg/mL
GITRL: 24 – 100,000 pg/mL
HVEM: 2 – 10,000 pg/mL
ICOS: 49 – 200,000 pg/mL
LAG-3: 122 – 500,000 pg/mL
PD-1: 24 – 100,000 pg/mL
PD-L1: 5 – 20,000 pg/mL
PD-L2: 49 – 200,000 pg/mL
TIM-3: 5 – 20,000 pg/mL
TLR-2: 73 – 300,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
HCKP1-11K-PX17	04061841002239

Documentation

Protocols

Title
Protocol- HCKP1-11K\|HCKP1-11K-PX17\|HCKP1-11K-PXBK17

MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Premixed 17-plex Panel 1 - Immuno-Oncology Multiplex Assay MSDS

职位
物料安全数据表 (MSDS)

种类

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Premixed Kits

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