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Recombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
Catalogue Number
492027
Brand Family
Calbiochem®
Synonyms
Heregulin-1
References
References
Meyer, D. et al. 1997. Development124, 3575. Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
ED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
Purity
>95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Meyer, D. et al. 1997. Development124, 3575. Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-June-2011 RFH
Synonyms
Heregulin-1
Description
Recombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered PBS, BSA.
Purity
>95% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Biological activity
ED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
Solubility
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Meyer, D. et al. 1997. Development124, 3575. Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
