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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

516562 Sigma-AldrichPertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

Pertussis Toxin, Salt-Free, Bordetella pertussis, CAS 70323-44-3, is a protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Islet-activating Protein

Recommended Products

概述

Replacement Information

重要规格表

CAS #
70323-44-3

价格及供货情况

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Description
Overview	A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number	516562
Brand Family	Calbiochem®
Synonyms	Islet-activating Protein

References
References	Fryer, M.W. 1992. Neurosci. Lett. 146, 84. Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69. Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301. Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735. Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684. Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479. Kaslow, H.R., et al. 1987. Biochemistry 26, 123. Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198. Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

References

Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

Product Information
CAS number	70323-44-3
ATP Competitive	N
Form	Salt-free lyophilized solid
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Biological activity	≤0.01 ng/ml. The activity is defined as the lowest concentration that promotes a clustering pattern in the CHO cell assay
Primary Target	ADP-ribosylation of guanine nucleotide-binding regulatory proteins
Purity	Five distinct bands by SDS-PAGE

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	XW5883750

Safety Information
R Phrase	R: 23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
S Phrase	S: 36/37/39-45 Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Toxic
Hazardous Materials Attention:	Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage	+2°C to +8°C
Do not freeze	Yes
Special Instructions	Following reconstitution, store in the refrigerator (4°C). For long term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for up to 6 months for use in CHO cell assays and for up to 2 months for use in non-cellular systems.
Canadian export regulations	Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
516562-50UGCN	04055977198188

Documentation

Pertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Pertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem 分析证书

标题	批号
516562	输入批号

参考

参考信息概述
Fryer, M.W. 1992. Neurosci. Lett. 146, 84. Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69. Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301. Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735. Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684. Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479. Kaslow, H.R., et al. 1987. Biochemistry 26, 123. Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198. Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-August-2008 RFH
Synonyms	Islet-activating Protein
Description	A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins G_i, G_o, and G_T. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of G_i proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1. S-1 (a protomer) is responsisble for the enzymatic activity of the toxin, and other subunits (2-5) form the B oligomer which is responsible for the toxin's binding to the cell surface. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow and Burns or Moss (see references).
Form	Salt-free lyophilized solid
CAS number	70323-44-3
RTECS	XW5883750
Purity	Five distinct bands by SDS-PAGE
Biological activity	≤0.01 ng/ml. The activity is defined as the lowest concentration that promotes a clustering pattern in the CHO cell assay
Solubility	Reconstitute with 500 µl sterile H₂O or aqueous buffer. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Special Instructions	Following reconstitution, store in the refrigerator (4°C). For long term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for up to 6 months for use in CHO cell assays and for up to 2 months for use in non-cellular systems.
Toxicity	Toxic
References	Fryer, M.W. 1992. Neurosci. Lett. 146, 84. Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69. Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301. Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735. Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684. Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479. Kaslow, H.R., et al. 1987. Biochemistry 26, 123. Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198. Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

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