数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CL07 Sigma-AldrichTMB Solution

This TMB Solution is validated for use in ELISA for the detection of TMB Solution.

TMB Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
Data Sheet

Recommended Products

概述

Replacement Information

价格及供货情况

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Description
Overview	A soluble, non-toxic, non-flammable horseradish peroxidase substrate.
Catalogue Number	CL07
Brand Family	Calbiochem®
Application Data	TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible. Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times. TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.

References

Product Information
CAS number	54827-17-7
Form	Liquid
Formulation	In a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
Quality Level	MQ100

Applications
Application Notes	ELISA
Application Comments	Product does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	DV2300000

Safety Information
R Phrase	R: 21/22-36/38 Harmful in contact with skin and if swallowed. Irritating to eyes and skin.
S Phrase	S: 26-36/37-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Harmful
Storage	+2°C to +8°C
Protect from Light	Protect from light
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
CL07-1000MLCN	04055977228496
CL07-100MLCN	04055977228489

Documentation

TMB Solution MSDS

职位
物料安全数据表 (MSDS)

TMB Solution 分析证书

标题	批号
CL07	输入批号

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	21-August-2007 RFH
Application	ELISA
Application Data	TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible. Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times. TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.
Description	A soluble, non-toxic, non-flammable horseradish peroxidase substrate.
Background	TMB (Soluble Substrate) is a non-carcinogenic chromophore for use in ELISA and other microwell applications. TMB (Soluble Substrate) contains 3,3',5,5'-tetramethylbenzidine formulated in a mildly acidic buffer with proprietary stabilizers. It is supplied as a one component liquid solution at a ready-to-use working dilution for application use. Unreacted substrate should be colorless or a very light-bluish-green prior to use. The substrate system develops a blue reaction product when reacted with peroxidase conjugates in microwell applications for visual reading at a wavelength of 370 nm and between 620 and 650 nm. For endpoint assays, the addition of an equal volume of 1N or 2N HCl, or 1N H2SO4 stops the reaction and turns the blue reaction product to a yellow reaction product and is read at a wavelength of 450 nm. Endpoint assays should be read between thirty and sixty minutes after addition of stopping solution. TMB (Soluble Substrate) produces a soluble reaction product that is not recommended for membrane or immunohistochemical applications, which require a precipitating reaction product.
Form	Liquid
Formulation	In a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
CAS number	54827-17-7
RTECS	DV2300000
Comments	Product does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.
Storage	Protect from light +2°C to +8°C
Do Not Freeze	Yes
Toxicity	Harmful

种类

Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Substrates for AP & HRP

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