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Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.
Catalogue Number
598502
Brand Family
Calbiochem®
Synonyms
ryNTR
References
References
Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1. Wang, P.F., et al. 1998. Biochemistry37, 16378. Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.
Form
Lyophilized solid
Formulation
Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.
Thioredoxin Reductase, Yeast, Recombinant, E. coli 分析证书
标题
批号
598502
参考
参考信息概述
Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1. Wang, P.F., et al. 1998. Biochemistry37, 16378. Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-June-2008 JSW
Synonyms
ryNTR
Description
Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.
Form
Lyophilized solid
Formulation
Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.
Purity
≥95% by SDS-PAGE
Specific activity
≥5 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.
Solubility
Aqueous buffers, pH 7.0
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.
Toxicity
Standard Handling
References
Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1. Wang, P.F., et al. 1998. Biochemistry37, 16378. Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
