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42600 Ultrafree-DA 离心超滤管

Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction

Ultrafree-DA 离心超滤管 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      概述

      Replacement Information
      描述
      产品目录编号42600
      商名
      • Ultrafree
      描述Ultrafree-DA 离心超滤管
      概述Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

      The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

      Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

      • Minimal hands-on time
      • 10-minute spin
      • Fully-functional DNA


      Performance
      Typical DNA Recoveries from Agarose Gels

      DNA Size (bp) Percent DNA Recoveries

      100 78
      700 71
      1000 77
      2027 47
      4361 35
      9416 32
      23130 29

      Protocol
      1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
      2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
      3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
      4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
      参考
      产品信息
      HS代码8421 29 90
      色码蓝色
      最高工作温度50 °C
      过滤器类型微孔滤膜
      Quality LevelMQ400
      应用
      应用Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction
      主要应用
      • DNA Extraction
      应用说明固定角度 Microfuge 转子，最大离心力为 5,000 x g
      生物信息
      无菌性非无菌
      理化信息
      孔径Not Applicable
      离心应用DNA Gel Extraction
      尺寸
      长度45 mm
      直径10.7 mm
      过滤面积0.2 cm²
      体积0.5 mL
      材料信息
      化学
      • Polypropylene (PP)
      装置材料
      • Polypropylene
      毒性学信息
      GHS安全信息
      安全信息
      产品使用声明
      储存和货运信息
      包装信息
      数量50
      运输信息
      补充信息
      规格
      Global Trade Item Number
      产品目录编号 GTIN
      42600 04053252743214

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      产品族

      从琼脂糖凝胶中回收 DNA

      Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin.
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      种类

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > Gel Extraction Centrifugal Filter Units