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Alternative to DFP and PMSF. An irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentration. Acts on C1r, C1s, Factor Xa, and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF.
Catalogue Number
178281
Brand Family
Calbiochem®
Synonyms
p-Amidinophenylmethylsulfonylfluoride, HCl
References
References
Cole, T.C., et al. 1989. Biochim. Biophys. Acta 990, 254. Laura, R., et al. 1980. Biochemistry19, 4859.
p-APMSF, Hydrochloride - CAS 74938-88-8 - Calbiochem 分析证书
标题
批号
178281
参考
参考信息概述
Cole, T.C., et al. 1989. Biochim. Biophys. Acta 990, 254. Laura, R., et al. 1980. Biochemistry19, 4859.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
15-April-2008 RFH
Synonyms
p-Amidinophenylmethylsulfonylfluoride, HCl
Description
An alternative to DFP and PMSF. Specific irreversible inhibitor of trypsin-like serine proteases. Inhibits trypsin and thrombin in equimolar concentrations. Acts on C1r, C1s, Factor Xa and plasmin, requiring a 5- to 10-fold excess for complete irreversible inhibition. Inhibitory activity is approximately 1000-fold greater than that of PMSF. Stability is pH dependent: t½ = 20 min at pH 6.0; 6 min at pH 7.0; 1 millisecond at pH 8.0.
Form
White solid
CAS number
74938-88-8
Purity
≥97% by HPLC
Solubility
H₂O (25 mg/ml)
Storage
Protect from moisture
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Unstable in solution; reconstitute just prior to use.
Toxicity
Standard Handling
References
Cole, T.C., et al. 1989. Biochim. Biophys. Acta 990, 254. Laura, R., et al. 1980. Biochemistry19, 4859.