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171373 Amphiregulin, Mouse, Recombinant

171373
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, mouse amphiregulin (AR) consisting of amino acids 94-191 and expressed in E. coli. Mouse AR shares 81% homology at the amino acid sequence level with rat AR. Amphiregulin, a growth inhibitor for certain tumor cells, also stimulates the proliferation of mammary epithelial cells, astrocytes, keratinocytes, glial cells, and fibroblasts.
Catalogue Number171373
Brand Family Calbiochem®
SynonymsSchwannoma-derived growth factor (SDGF)
References
ReferencesNormanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.
Product Information
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Applications
Biological Information
Biological activityED₅₀ = 5-20 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Amphiregulin, Mouse, Recombinant Certificates of Analysis

TitleLot Number
171373

References

Hivatkozások áttekintése
Normanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-September-2011 RFH
SynonymsSchwannoma-derived growth factor (SDGF)
DescriptionRecombinant, mouse amphiregulin (AR) consisting of amino acids 94-191 and expressed in E. coli. Mouse AR shares 81% homology at the amino acid sequence level with rat AR. Amphiregulin, a growth inhibitor for certain tumor cells, also stimulates the proliferation of mammary epithelial cells, astrocytes, keratinocytes, glial cells, and fibroblasts.
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 5-20 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesNormanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.