217575 Anti-CD4 Mouse mAb (QS4120)

217575
  
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      Áttekintés

      Replacement Information

      Kulcsspecifikációk táblázata

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      Description
      Overview

      This product has been discontinued.





      Recognizes domain 1 of CD4.
      Catalogue Number217575
      Brand Family Calbiochem®
      References
      ReferencesHealey, D., et al. 1990. J. Exp. Med. 172, 1233.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Preservative0.5 mg/ml gentamycin sulfate
      Applications
      Adatbeállítási hiba. A pagelet <collection_feature_application_id-2D PAGE> nem található.
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Application Notes2D Gel Electrophoresis (see comments)
      ELISA (see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Application CommentsFACS was performed on 5x105 HPB-MLT human tumor cells and analyzed by flow cytometry. Optimal binding was obtained at 5 µg/ml using goat anti-mouse IgG, FITC conjugated, as the secondary antibody. This antibody has also been reported to work for 2D gel electrophoresis, ELISA, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenCD4+ transfectant/human CEM
      ImmunogenHuman
      CloneQS4120
      HostMouse
      Purity≥95%
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD4 Mouse mAb (QS4120) Certificates of Analysis

      TitleLot Number
      217575

      References

      Hivatkozások áttekintése
      Healey, D., et al. 1990. J. Exp. Med. 172, 1233.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      Application2D Gel Electrophoresis (see comments)
      ELISA (see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes domain 1 of CD4.
      BackgroundThe amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120 and this antibody will block binding of HIV-1 gp120 to CD4. It also blocks HLA Class II Rosette formation.
      HostMouse
      Immunogen speciesHuman
      ImmunogenCD4+ transfectant/human CEM
      CloneQS4120
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95%
      Preservative0.5 mg/ml gentamycin sulfate
      CommentsFACS was performed on 5x105 HPB-MLT human tumor cells and analyzed by flow cytometry. Optimal binding was obtained at 5 µg/ml using goat anti-mouse IgG, FITC conjugated, as the secondary antibody. This antibody has also been reported to work for 2D gel electrophoresis, ELISA, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesHealey, D., et al. 1990. J. Exp. Med. 172, 1233.