217952 Anti-CD95L Mouse mAb (Alf-2.1a) Fluorescein Conjugate

217952
  
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      Áttekintés

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      Description
      Overview

      This product has been discontinued.





      Recognizes membrane bound and soluble human Fas ligand in KFL9 cells.
      Note: 1 T = 1 test.
      Catalogue Number217952
      Brand Family Calbiochem®
      SynonymsAnti-Fas Ligand, FITC
      References
      ReferencesSuda, T., et al. 1997. J. Exp. Med. 186, 2045.
      Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 5% glycerol, 0.2% BSA, pH 7.5.
      Negative controlHPB-MLT cells
      Positive controlKFL9 cells, full length CD95L transfectants
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Flow Cytometry
      Neutralization Studies
      Application NotesFlow Cytometry (1:50, 80 µl/test)
      Neutralization Studies (see comments)
      Application CommentsThis antibody recognizes both membrane-bound and soluble Fas ligand and can block Fas ligand activity. FACS was performed on 5x105 KFL9 cells and analyzed by flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogensoluble human Fas ligand
      ImmunogenHuman
      CloneAlf-2.1a
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD95L Mouse mAb (Alf-2.1a) Fluorescein Conjugate Certificates of Analysis

      TitleLot Number
      217952

      References

      Hivatkozások áttekintése
      Suda, T., et al. 1997. J. Exp. Med. 186, 2045.
      Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-Fas Ligand, FITC
      ApplicationFlow Cytometry (1:50, 80 µl/test)
      Neutralization Studies (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~40 kDa Fas ligand (CD95L) protein.
      BackgroundHuman Fas ligand (CD95L) is a ~40 kDa type II membrane protein that induces apoptosis in cells expressing Fas (CD95). Fas ligand is one of the major effectors of CD8+ cytotoxic T lymphocytes and NK activity. Loss of Fas ligand leads to lymphoproliferative and autoimmune disease.
      HostMouse
      Immunogen speciesHuman
      Immunogensoluble human Fas ligand
      CloneAlf-2.1a
      IsotypeIgG₁
      Specieshuman
      Positive controlKFL9 cells, full length CD95L transfectants
      Negative controlHPB-MLT cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 5% glycerol, 0.2% BSA, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody recognizes both membrane-bound and soluble Fas ligand and can block Fas ligand activity. FACS was performed on 5x105 KFL9 cells and analyzed by flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesSuda, T., et al. 1997. J. Exp. Med. 186, 2045.
      Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.