218794 Anti-Caspase-9 (1-134) Rabbit pAb

218794
  
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      Áttekintés

      Replacement Information

      Kulcsspecifikációk táblázata

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~46 kDa latent form of caspase-9 in heat shocked HeLa cells and the ~37 kDa, ~18 kDa (weakly), and ~10 kDa processed forms of casapase-9.
      Catalogue Number218794
      Brand Family Calbiochem®
      SynonymsAnti-ICE-LAP6, Anti-Mch6, Anti-APAF-3, Anti-CASP9
      Application Data
      Detection of human caspase-9 by immunoblotting. Samples: Whole cell lysates from Jurkat cells left untreated (lane 1) and apoptosis-inducing agents (lane 2). Primary antibody: Anti-Caspase-9 (1-134) Rabbit pAb (Cat. No. 218794) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Garrido, C., et al. 1999. FASEB J. 13, 2061.
      Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeat-shocked HeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500)
      Application CommentsThis antibody recognizes both active caspase-9 enzyme and pro-caspase-9. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena recombinant protein containing amino acids 1-134 of human caspase-9 fused to a His•Tag® sequence
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Caspase-9 (1-134) Rabbit pAb Certificates of Analysis

      TitleLot Number
      218794

      References

      Hivatkozások áttekintése
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Garrido, C., et al. 1999. FASEB J. 13, 2061.
      Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-ICE-LAP6, Anti-Mch6, Anti-APAF-3, Anti-CASP9
      ApplicationImmunoblotting (1:500)
      Application Data
      Detection of human caspase-9 by immunoblotting. Samples: Whole cell lysates from Jurkat cells left untreated (lane 1) and apoptosis-inducing agents (lane 2). Primary antibody: Anti-Caspase-9 (1-134) Rabbit pAb (Cat. No. 218794) (2 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~46 kDa, latent, the ~34-37 kDa active large subunit, and an ~18 kDa (weakly) and ~10 kDa (weakly) intermediates of caspase-9.
      BackgroundCaspase-9 is activated by the proteolysis of pro-caspase-9 by CPP32 (caspase-3) and granzyme B (caspase-8). It is composed of a 34-37 kDa large subunit and a 10 kDa small subunit.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein containing amino acids 1-134 of human caspase-9 fused to a His•Tag® sequence
      IsotypeIgG
      Speciesbovine, chicken, guinea pig, human, monkey, mouse, rat, sheep
      Positive controlHeat-shocked HeLa cells
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsThis antibody recognizes both active caspase-9 enzyme and pro-caspase-9. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Garrido, C., et al. 1999. FASEB J. 13, 2061.
      Krajewski, S., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 5752.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.