324610 Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb

324610
  
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      Áttekintés

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~160 kDa EEA1 protein.
      Catalogue Number324610
      Brand Family Calbiochem®
      SynonymsAnti-EEA1
      Application Data
      Detection of mouse early endosomal antigen 1 by immunoblotting. Samples: Whole tissue extract from mouse brain left untreated (lane 1) and treated with neutralizing peptide (lane 2). Primary antibody: Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb (Cat. No. 324610) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesMills, I.G., et al. 1998. Curr. Biol. 8, 881.
      Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (1 µg/ml)
      Immunofluorescence (4 µg/ml, see comments)
      Application CommentsImmunofluorescence localization of EEA1 in HeLa cells shows a punctate staining pattern consistent with the cytoplasmic distribution of endosomes. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1391-1410 of human EEA1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb Certificates of Analysis

      TitleLot Number
      324610

      References

      Hivatkozások áttekintése
      Mills, I.G., et al. 1998. Curr. Biol. 8, 881.
      Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      SynonymsAnti-EEA1
      ApplicationImmunoblotting (1 µg/ml)
      Immunofluorescence (4 µg/ml, see comments)
      Application Data
      Detection of mouse early endosomal antigen 1 by immunoblotting. Samples: Whole tissue extract from mouse brain left untreated (lane 1) and treated with neutralizing peptide (lane 2). Primary antibody: Anti-Early Endosomal Antigen 1 (1391-1410) Rabbit pAb (Cat. No. 324610) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~160 kDa EEA1 protein.
      BackgroundEEA1 is a membrane-bound protein specific to the early endosome that is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments that fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Membrane association of EEA1 is dependent on PI-3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. EEA1 contains C-terminal zinc-finger-like domains similar to those found in nucleic acid binding proteins, implicating its role in membrane trafficking.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1391-1410 of human EEA1
      IsotypeIgG
      Speciescanine, hamster, human, mouse, rat
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunofluorescence localization of EEA1 in HeLa cells shows a punctate staining pattern consistent with the cytoplasmic distribution of endosomes. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMills, I.G., et al. 1998. Curr. Biol. 8, 881.
      Stenmark, H., et al. 1996. J. Biol. Chem. 271, 24048.