324756 Anti-Elastin Rabbit pAb

324756
  
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Elmentette a ()-t
 
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      Áttekintés

      Replacement Information

      Kulcsspecifikációk táblázata

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes insoluble elastin and tropoelastin.
      Catalogue Number324756
      Brand Family Calbiochem®
      References
      ReferencesWrenn, D.S., and Mecham, R.P. 1987. Methods Enzymol. 144, 246.
      Mecham, R.P., and Lange, G. 1982. Methods Enzymol. 82 (Pt. A), 744.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoprecipitation
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (1:1000-1:2000)
      Immunoblotting (1:200-1:500)
      Immunohistochemistry (1:50-1:100, see comments)
      Immunoprecipitation (1:100-1:200)
      Application CommentsReactivity is weaker in non-human species. Antigenicity of elastic fiber components can be enhanced by treating tissue sections or fixed cell preparations with 6 M guanidine HCl, 20 mM Tris, 50 mM DTT, pH 8.0, for 15 min. Wash slides with 20 mM Tris, pH 8.0, then treat with 100 mM iodoacetamide in the dark for 15 min. Wash again in 20 mM Tris and stain using normal protocols. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenthe α-elastin fraction of insoluble elastin purified from human aorta
      ImmunogenHuman
      HostRabbit
      IsotypelgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 50% glycerol in PBS containing 0.01% sodium azide. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -20°C or for up to 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Elastin Rabbit pAb Certificates of Analysis

      TitleLot Number
      324756

      References

      Hivatkozások áttekintése
      Wrenn, D.S., and Mecham, R.P. 1987. Methods Enzymol. 144, 246.
      Mecham, R.P., and Lange, G. 1982. Methods Enzymol. 82 (Pt. A), 744.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-August-2007 RFH
      ApplicationELISA (1:1000-1:2000)
      Immunoblotting (1:200-1:500)
      Immunohistochemistry (1:50-1:100, see comments)
      Immunoprecipitation (1:100-1:200)
      DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes insoluble elastin and tropoelastin.
      HostRabbit
      Immunogen speciesHuman
      Immunogenthe α-elastin fraction of insoluble elastin purified from human aorta
      IsotypelgG
      Speciesbovine, canine, human, rabbit, not rat
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsReactivity is weaker in non-human species. Antigenicity of elastic fiber components can be enhanced by treating tissue sections or fixed cell preparations with 6 M guanidine HCl, 20 mM Tris, 50 mM DTT, pH 8.0, for 15 min. Wash slides with 20 mM Tris, pH 8.0, then treat with 100 mM iodoacetamide in the dark for 15 min. Wash again in 20 mM Tris and stain using normal protocols. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 50% glycerol in PBS containing 0.01% sodium azide. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -20°C or for up to 3 months at 4°C.
      Toxicity Standard Handling
      ReferencesWrenn, D.S., and Mecham, R.P. 1987. Methods Enzymol. 144, 246.
      Mecham, R.P., and Lange, G. 1982. Methods Enzymol. 82 (Pt. A), 744.