341282 Anti-FADD (125-140) Rabbit pAb

341282
  
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      Recognizes the ~28 kDa FADD protein.
      Catalogue Number341282
      Brand Family Calbiochem®
      SynonymsAnti-MORT1, Anti-Fas-Associating Death Domain Containing Protein
      Application Data
      Detection of human FADD by immunoblotting. Samples: Molecular weight marker (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-FADD (125-140) Rabbit pAb (Cat. No. 341282) (1:1000). Detection: chemiluminescence.
      References
      ReferencesIrmler, M., et al. 1997. Nature 388, 190.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Varfolomeev, E.E., et al. 1996. J. Exp. Med. 183, 1271.
      Chinnaiyan, A.M., et al. 1995. Cell 81, 505.
      Enari, M., et al. 1995. Nature 375, 78.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000-1:4000, colorimetric)
      Application CommentsThis antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)KIDSIEDRYPRNLTER] corresponding to amino acids 125-140 of human FADD, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-FADD (125-140) Rabbit pAb Certificates of Analysis

      TitleLot Number
      341282

      References

      Hivatkozások áttekintése
      Irmler, M., et al. 1997. Nature 388, 190.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Varfolomeev, E.E., et al. 1996. J. Exp. Med. 183, 1271.
      Chinnaiyan, A.M., et al. 1995. Cell 81, 505.
      Enari, M., et al. 1995. Nature 375, 78.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-April-2008 JSW
      SynonymsAnti-MORT1, Anti-Fas-Associating Death Domain Containing Protein
      ApplicationImmunoblotting (1:1000-1:4000, colorimetric)
      Application Data
      Detection of human FADD by immunoblotting. Samples: Molecular weight marker (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-FADD (125-140) Rabbit pAb (Cat. No. 341282) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa FADD protein.
      BackgroundFADD is a cytosolic protein that serves as a signal transducer of Fas-induced apoptosis. FADD contains a death domain homologous to Fas and interacts with Fas to initiate apoptosis. Through its amino-terminal death effector domain, FADD binds cytosolic proteins such as TRADD or caspase 8 (FLICE) to transduce the death signal.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(C)KIDSIEDRYPRNLTER] corresponding to amino acids 125-140 of human FADD, conjugated to KLH
      IsotypeIgG
      Specieshuman, monkey, mouse (weakly), rat (weakly)
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsThis antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesIrmler, M., et al. 1997. Nature 388, 190.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Varfolomeev, E.E., et al. 1996. J. Exp. Med. 183, 1271.
      Chinnaiyan, A.M., et al. 1995. Cell 81, 505.
      Enari, M., et al. 1995. Nature 375, 78.