Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

06-1025 Anti-NET39 Antibody

06-1025
100 µg  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1025
DescriptionAnti-NET39 Antibody
Alternate Names
  • phosphatidic acid phosphatase type 2 domain containing 3
  • chromosome 9 open reading frame 67
  • probable lipid phosphate phosphatase PPAPDC3
  • Phosphatidic acid phosphatase type 2 domain-containing protein 3
Background InformationNET39 (nuclear envelope transmembrane protein 39) also known as Probable lipid phosphate phosphatase PPAPDC3, has been shown to play a part in regulation of signaling and gene expression. By effecting mTOR signaling, NET39 has a negative regulatory effect on myoblast differentiation. It is predominantly expressed in cardiac and skeletal muscle. During myoblast differentiation, NET39 is highly upregulated. NET39 has been found to be an important part of regulation for myogenesis and might also be involved in muscle homeostasis.
References
Product Information
FormatAffinity Purified
Control
  • Rat liver tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-NET39 Antibody is a Rabbit Polyclonal Antibody for detection of NET39 has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected NET39 in A431 and HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human NET39 at the cytoplasmic domain.
EpitopeCytoplasmic domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes NET39 at the cytoplasmic domain.
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPAPDC3
  • NET39
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling. Has no detectable enzymatic activity (By similarity).

SUBUNIT STRUCTURE: Homo and heterooligomer. Interacts with MTOR; controls MTOR-dependent IGF2 expression during myoblast differentiation (By similarity).

SUBCELLULAR LOCATION: Nucleus envelope. Endoplasmic reticulum membrane. Membrane; Multi-pass membrane protein. Note: Both the N- and C-terminal are exposed to the cytoplasm/nucleoplasm (By similarity).

SEQUENCE SIMILARITIES: Belongs to the PA-phosphatase related phosphoesterase family.

SEQUENCE CAUTION: The sequence CAI16274.1 differs from that shown. Reason: Erroneous gene model prediction.
Molecular Weight ~ 29 kDa observed. The band at ~23 kDa is uncharacterized, but may represent a possible isoform of this protein . (Malik, P., et al. (2010).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat liver tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NET39 on 10 µg of rat liver tissue lysate. Note: Western blot performed without Tween.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
06-1025 04053252414046

Documentation

Anti-NET39 Antibody Certificates of Analysis

TitleLot Number
Anti-NET39 - NRG1818958 NRG1818958

References

Reference overviewPub Med ID
Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.
Zuleger, N; Boyle, S; Kelly, DA; de las Heras, JI; Lazou, V; Korfali, N; Batrakou, DG; Randles, KN; Morris, GE; Harrison, DJ; Bickmore, WA; Schirmer, EC
Genome biology  14  R14  2013

Kivonat megmutatása
23414781 23414781