218820 Caspase-2 Substrate IV, Colorimetric

218820
  
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      Description
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      This product has been discontinued.





      A colorimetric substrate for the detection of caspase-2 (ICH-1). Cleavage is monitored colorimetrically at ~405 nm.
      Catalogue Number218820
      Brand Family Calbiochem®
      SynonymsAc-VDVAD-pNA
      References
      ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
      Product Information
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₂₉H₄₂N₇O₁₂
      Chemical formulaC₂₉H₄₂N₇O₁₂
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide SequenceAc-Val-Asp-Val-Ala-Asp-pNA
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Caspase-2 Substrate IV, Colorimetric Certificates of Analysis

      TitleLot Number
      218820

      References

      Hivatkozások áttekintése
      Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      SynonymsAc-VDVAD-pNA
      DescriptionA colorimetric substrate for the detection of caspase-2 (ICH-1) activity (Km = 53 µM). May also serve as a substrate for caspase-3 (Km = 67 µM) and caspase-7 (Km = 200 µM). Cleavage is monitored by an increase in absorbance at 405 nm.
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Recommended reaction conditions100 mM NaOAc, pH 6.2, 20% (v/v) glycerol, 5 mM DTT, 0.5 mM EDTA, 0.1% (w/v) BSA, 3-200 ng purified caspase-2, 10-100 mM Ac-VDVAD-pNA.
      Chemical formulaC₂₉H₄₂N₇O₁₂
      Peptide SequenceAc-Val-Asp-Val-Ala-Asp-pNA
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Protect from moisture
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Standard Handling
      ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.