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218782 Caspase Substrate Set II, Fluorogenic

218782
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
OverviewContains 1 mg each of Caspase-1 Substrate III, Ac-YVAD-AMC (Cat. No. 400020); Caspase-2 Substrate I, Z-VDVAD-AFC (Cat. No. 218740); Caspase-3 Substrate II, Ac-DEVD-AMC (Cat. No. 235425); Caspase-4 Substrate II, Ac-LEVD-AFC (Cat. No. 218748); Caspase-5 Substrate II, Ac-WEHD-AFC (Cat. No. 218754); Granzyme B Substrate II, Z-IETD-AFC (Cat. No. 268059); Caspase-9 Substrate I, Ac-LEHD-AFC (Cat. No. 218765); 5 mg of Caspase-6 Substrate I, Ac-VEID-AMC (Cat. No. 218760); 10 mg of AMC Reference Standard (Cat. No. 164545); and 50 mg of AFC Reference Standard (Cat. No. 164580). Supplied with a data sheet.
Catalogue Number218782
Brand Family Calbiochem®
References
ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702. Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Nicholson, D.W., et al. 1995. Nature 376, 37. Thornberry, N.A., et al. 1992. Nature 356, 768. Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
Product Information
FormSolid
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
218782 0

Documentation

References

Hivatkozások áttekintése
Humke, E.W., et al. 1998. J. Biol. Chem. 273, 15702. Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Nicholson, D.W., et al. 1995. Nature 376, 37. Thornberry, N.A., et al. 1992. Nature 356, 768. Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2008 JSW
DescriptionApoptosis is a normal process in development and morphogenesis. However, improperly regulated apoptosis can contribute to several pathological conditions including cancer, Alzheimer's disease, spinal muscular atrophy, ischemic cardiac damage, and autoimmune syndromes. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. Receptor-mediated apoptosis involves the catalysis of preexisting intracellular caspase proenzymes into activated caspases (cysteine-containing aspartate-specific proteases). A distinctive feature of caspases is the requirement of an aspartic acid residue in the substrate P1 position. The P4 residue is important in substrate recognition and specificity. For example, caspase-1 prefers a hydrophobic tyrosine residue in the P4 position, but caspase-3 prefers an anionic aspartic residue. Caspase activity can be assayed using a fluorophore (AFC or AMC). Liberation of the fluorophore from the substrate is monitored to determine caspase activity. Supplied with a data sheet.
FormSolid
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Toxicity Multiple Toxicity Values, refer to MSDS
ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702. Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Nicholson, D.W., et al. 1995. Nature 376, 37. Thornberry, N.A., et al. 1992. Nature 356, 768. Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.