233903 Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem

233903
  
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      Áttekintés

      Replacement Information

      Kulcsspecifikációk táblázata

      Empirical FormulaCAS #
      C₂₆H₂₁N₃O₂ 50909-86-9
      Description
      OverviewA highly sensitive and a specific intracellular luminophore that is useful for measuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA. Exhibits nearly 16-fold greater luminescence intensity (emission maximum ~466 nm; half-total time of 0.6 - 1.2 sec) as compared to the native Coelenterazine (Cat. No. 233900).
      Catalogue Number233903
      Brand Family Calbiochem®
      SynonymsCLZ-h, 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
      References
      ReferencesNebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
      Shimomura, O. 1997. Biochem. J. 326, 297.
      Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
      Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
      Shimomura, O., et al. 1993. Biochem. J. 296, 549.
      Shimomura, O., et al. 1993. Cell Calcium 14, 373.
      Shimomura, O., et al. 1989. Biochem. J. 261, 913.
      Product Information
      CAS number50909-86-9
      ATP CompetitiveN
      FormYellow brown solid
      Hill FormulaC₂₆H₂₁N₃O₂
      Chemical formulaC₂₆H₂₁N₃O₂
      ReversibleN
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Primary TargetMeasuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA.
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot, purge with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      233903

      References

      Hivatkozások áttekintése
      Nebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
      Shimomura, O. 1997. Biochem. J. 326, 297.
      Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
      Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
      Shimomura, O., et al. 1993. Biochem. J. 296, 549.
      Shimomura, O., et al. 1993. Cell Calcium 14, 373.
      Shimomura, O., et al. 1989. Biochem. J. 261, 913.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-April-2011 RFH
      SynonymsCLZ-h, 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
      DescriptionA membrane-permeable, highly sensitive, specific, intracellular luminophore that is useful for measuring changes in Ca2+. For the measurement of Ca2+ levels coelenterazine is used in cells that have been transfected with apoaequorin cDNA. In this system, coelenterazine is required for the regeneration of aequorin, a protein that emits light in the presence of calcium, from apoaequorin produced in cells. The luminescence intensity appears to be directly proportional to the Ca2+ concentration. Coelentrazine-h exhibits an approximate 16-fold greater luminescence intensity (emission max.: ~466 nm; half-time total of 0.6-1.2 s) as compared to the native Coelenterazine (Cat. No. 233900). Has been used to measure intracellular Ca2+ signals in Dictyostelium discoideum chemotaxis and in plant wound healing.
      FormYellow brown solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number50909-86-9
      Chemical formulaC₂₆H₂₁N₃O₂
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityEthanol (10 mg/ml) or Methanol (10 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot, purge with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesNebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
      Shimomura, O. 1997. Biochem. J. 326, 297.
      Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
      Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
      Shimomura, O., et al. 1993. Biochem. J. 296, 549.
      Shimomura, O., et al. 1993. Cell Calcium 14, 373.
      Shimomura, O., et al. 1989. Biochem. J. 261, 913.