251756 DARPP-32, Phospho-, Rat, Recombinant, E. coli

251756
  
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      Áttekintés

      Replacement Information
      Description
      OverviewRecombinant, rat DARPP-32 expressed in E. coli and phosphorylated on Thr34 by cAMP-dependent protein kinase (PKA). Acts as a potent inhibitor of protein phosphatase-1 (PP1; IC50 = 1 nM). It is dephosphorylated and inactivated by PP2A and PP2B.
      Catalogue Number251756
      Brand Family Calbiochem®
      SynonymsPhospho-dopamine- and cAMP-regulated Phosphoprotein
      References
      ReferencesNishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Nishi, A., et al. 1999. J. Neurochem. 72, 2015. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Desdouits, F., et al. 1995. Biochem. Biophys. 206, 652. Hemmings, H.C., and Greengard, P. 1986. J. Neurosci. 6, 1469. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99.
      Product Information
      FormWhite lyophilized solid
      Hygroscopic Hygroscopic
      Applications
      Biological Information
      Primary TargetPP1
      Primary Target IC<sub>50</sub>1 nM
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DARPP-32, Phospho-, Rat, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      251756

      References

      Hivatkozások áttekintése
      Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Nishi, A., et al. 1999. J. Neurochem. 72, 2015. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Desdouits, F., et al. 1995. Biochem. Biophys. 206, 652. Hemmings, H.C., and Greengard, P. 1986. J. Neurosci. 6, 1469. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-June-2008 RFH
      SynonymsPhospho-dopamine- and cAMP-regulated Phosphoprotein
      DescriptionRecombinant, rat DARPP-32 expressed in E. coli and phosphorylated on Thr34 by cAMP-dependent protein kinase (PKA). Serves as an important component of dopaminergic function in the brain. Acts as a potent inhibitor of protein phosphatase 1 (IC50 = ~1 nM). It is dephosphorylated and inactivated by calcineurin and protein phosphatase 2A (PP2A) in vitro. Also, found in the kidney where it participates in the regulation of the phosphorylation state of Na+,K+ -ATPase and control of salt balance. In adipocytes, it plays a role in adipogenesis and insulin signaling by maintaining low PP1 activity.
      FormWhite lyophilized solid
      Purity≥95% by SDS-PAGE
      Solubility≥2 mg/ml in 50 mM Tris-HCl buffer, pH 7.0
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesNishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Nishi, A., et al. 1999. J. Neurochem. 72, 2015. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Desdouits, F., et al. 1995. Biochem. Biophys. 206, 652. Hemmings, H.C., and Greengard, P. 1986. J. Neurosci. 6, 1469. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99.