251755 DARPP-32, Rat, Recombinant, E. coli

251755
  
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      Áttekintés

      Replacement Information
      Description
      OverviewRecombinant, rat DARPP-32 expressed in E. coli. DARPP-32 is an important component of dopaminergic function in the brain. Acts as an intracellular third messenger. Phosphorylation of DARPP-32 at Thr34 by cAMP-dependent protein kinase (PKA), results in its conversion to a potent inhibitor of protein phosphatase-1 (PP1; IC50 = 1-2 µM). Conversely, Thr34-phosphorylated DARPP-32 is dephosphorylated and inactivated in vitro by calcineurin (PP2B) and protein phosphatase-2A (PP2A). In resting neostratial slices, cyclin-dependent kinase 5 (Cdk5) phosphorylates DARPP-32 at Thr35 thereby reducing the efficacy of dopaminergic signaling.
      M.W. 32,000 (by SDS-PAGE).
      Catalogue Number251755
      Brand Family Calbiochem®
      SynonymsDopamine- and cAMP-regulated Phosphoprotein
      References
      ReferencesHiguchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.
      Product Information
      FormWhite lyophilized solid
      Hygroscopic Hygroscopic
      Applications
      Biological Information
      Primary TargetPP1
      Primary Target IC<sub>50</sub>1-2 µM
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DARPP-32, Rat, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      251755

      References

      Hivatkozások áttekintése
      Higuchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.

      Citations

      Titulus
    • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-December-2008 RFH
      SynonymsDopamine- and cAMP-regulated Phosphoprotein
      DescriptionRecombinant, rat DARPP-32 expressed in E. coli. DARPP-32 (dopamine- and cAMP-regulated phosphoprotein) is an important component of dopaminergic function in the brain. Acts as an intracellular third messenger. Phosphorylation of DARPP-32 at Thr34 by cAMP-dependent protein kinase (PKA) converts it into a potent inhibitor of protein phosphatase 1 (IC50 = ~1 µM). However, phosphorylation at Thr35 by Cdk5 converts it into a competitive inhibitor of PKA. It is dephosphorylated and inactivated by calcineurin and protein phosphatase 2A (PP2A) in vitro. Also, found in the kidney where it participates in the regulation of the phosphorylation state of Na+, K+ -ATPase and control of salt balance. In adipocytes, it plays a role in adipogenesis and insulin signaling by maintaining low PP1 activity.
      FormWhite lyophilized solid
      Purity≥95% by SDS-PAGE
      Solubility50 mM Tris-HCl, pH 7.0
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesHiguchi, E., et al. 2000. Eur. J. Pharmacol. 408, 107. Nishi, A., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12840. Yang, J., et al. 2000. J. Biol. Chem. 275, 22635. Greengard, P., et al. 1999. Neuron 23, 435. Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870. Oliver, C.J., and Shenolikar, S. 1998. Front. Biosci. 3, 961. Fryckstedt, J., et al. 1992. J. Auton. Pharmacol. 12, 183. Hemmings, H.C., Jr., et al. 1984. J. Neurosci. 4, 99. Hemmings, H.C., et al. 1984. Nature 310, 503.
      Citation
    • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.