324845 Endothelial Cell Growth Supplement, Bovine Neural Tissue

324845
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Katalógusszám ElérhetőségCsomagolás Menny./csomag Ár Mennyiség
324845-15MG
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      Description
      OverviewNative endothelial cell growth supplement from bovine neural tissue. A cell culture-medium supplement screened for contamination by bacteria, fungi, and mycoplasma. Mitogenic activity thought to be physically, chemically, and biologically distinct from that of FGF. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers.
      Catalogue Number324845
      Brand Family Calbiochem®
      SynonymsECGS
      References
      ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
      Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
      Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
      Olander, J., et al. 1980. In Vitro 16, 209.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20/21/22-61

      Harmful by inhalation, in contact with skin and if swallowed.
      May cause harm to the unborn child.
      S PhraseS: 22-36/37/39-45

      Do not breathe dust.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful & Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Endothelial Cell Growth Supplement, Bovine Neural Tissue Certificates of Analysis

      TitleLot Number
      324845

      References

      Hivatkozások áttekintése
      Blann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
      Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
      Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
      Olander, J., et al. 1980. In Vitro 16, 209.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-June-2008 RFH
      SynonymsECGS
      DescriptionNative endothelial cell growth supplement from bovine neural tissue. A cell-culture-medium supplement for the growth and maintenance of many cell types. Growth promoting activities have been described for human endothelial cells, bovine endothelial cells, and mouse Balb/c fibroblasts. Can also substitute for feeder cells in support of the clonal growth of hybridomas. Mitogenic activity of the preparation is thought to be physically, chemically, and biologically distinct from that of FGF. Media containing 50-150 μg/ml is recommended for culturing vascular endothelial cells. Acts as a superior cloning factor for the isolation and growth of hybridoma cells and may eliminate the need for feeder cell layers. Screened for contamination by bacteria, fungi, and mycoplasma.
      FormLyophilized
      FormulationLyophilized from sterile-filtered NaCl containing streptomycin sulfate.
      Concentration Label Please refer to vial label for lot-specific concentration
      Biological activitygrowth-promoting activity confirmed with fetal bovine endothelial cells
      SolubilityUsing aseptic technique, add 1-5 ml sterile serum-free medium and solubilize by gentle aspiration with a sterile pipette.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Harmful & Carcinogenic / Teratogenic
      ReferencesBlann, A.D. 1991. Cell Biol. Int. Rep. 15, 493.
      Jassal, D., et al. 1991. In Vitro Cell. Dev. Biol. 27A, 625.
      Tanswell, A.K., et al. 1991. J. Dev. Physiol. 15, 199.
      Olander, J., et al. 1980. In Vitro 16, 209.