330657 Estrogen Receptorβ, Human, Recombinant, S. frugiperda

330657
  
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      Áttekintés

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human estrogen receptor-β expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-α, especially in the DNA binding protein domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.

      Aliquot in individual vials for daily use. Do not add any dilution buffers, as these may cause aggregation of the ER.
      Catalogue Number330657
      Brand Family Calbiochem®
      References
      ReferencesKuiper, G.G., et al. 1997. Endocrinology 138, 863.
      Paech, K., et al. 1997. Science 277, 1508.
      Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
      Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of estrogen receptor that will bind 1.0 pmol ³H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay.
      FormLiquid
      FormulationIn 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX.
      Applications
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥5000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not add any dilution buffers, as these may cause aggregation of the ER.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Estrogen Receptorβ, Human, Recombinant, S. frugiperda MSDS

      Title

      Safety Data Sheet (SDS) 

      Estrogen Receptorβ, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      330657

      References

      Hivatkozások áttekintése
      Kuiper, G.G., et al. 1997. Endocrinology 138, 863.
      Paech, K., et al. 1997. Science 277, 1508.
      Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
      Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2007 JSW
      DescriptionRecombinant, human estrogen receptor-β expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-α, especially in the DNA binding domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.
      FormLiquid
      FormulationIn 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥80% by SDS-PAGE
      Specific activity≥5000 units/mg protein
      Unit definitionOne unit is defined as the amount of estrogen receptor that will bind 1.0 pmol ³H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not add any dilution buffers, as these may cause aggregation of the ER.
      Toxicity Standard Handling
      ReferencesKuiper, G.G., et al. 1997. Endocrinology 138, 863.
      Paech, K., et al. 1997. Science 277, 1508.
      Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
      Obourn, J.D., et al. 1993. Biochemistry 32, 6229.

      Kapcsolódó termékek és alkalmazások

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      Kategóriák

      Life Science Research > Proteins and Enzymes > Other Proteins