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71087 KOD XL DNA Polymerase

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KatalógusszámCsomagolás Menny./csomag
71087-3 Üvegpalack 250 u
71087-4 Üvegpalack 1250 u
OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.
KOD XL DNA Polymerase* is an optimized blend of KOD DNA Polymerase and a mutant form of KOD that is deficient in 3′→5′ exonuclease activity (Nishioka 2002). This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of derivatized dNTPs in PCR amplicons (Sawai 2002, Sawai 2001). KOD XL DNA Polymerase generates a mixture of PCR products with blunt and 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.

Source Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli (wild type and exonuclease-deficient forms)
Concentration 2.5 U/µl
Endonuclease None detected
Nicking activity None detected
Amplification effiency Functional PCR
Storage –20°C

*Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Licensed under US Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Catalogue Number71087 Brand Family Novagen®
Features and benefits

  • Ideal for amplification of large DNA fragments from purified DNA or crude samples
  • Amplifies DNA templates up to 30 kbp
  • Successfully amplifies GC-rich sequences
  • Efficiently incorporates derivatized dNTPs
ReferencesNishioka, M., et al. 2002. J. Biotechnol. 88, 141. Sawai, H., et al. 2002. Bioconjugate Chem. 13, 309. Sawai, H., et al. 2001. Chem. Commun. 24, 2604.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.
DeclarationManufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process for research use in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Katalógusszám GTIN
71087-3 04055977271737
71087-4 04055977271744


KOD XL DNA Polymerase Certificates of Analysis

TitleLot Number


Hivatkozások áttekintése
Nishioka, M., et al. 2002. J. Biotechnol. 88, 141. Sawai, H., et al. 2002. Bioconjugate Chem. 13, 309. Sawai, H., et al. 2001. Chem. Commun. 24, 2604.


High fidelity gene amplification
PCR Protocols and Guides - Simplify your gene discovery
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression


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  • User Protocols

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