IMMUNOLOGY 2017

Join us at IMMUNOLOGY 2017 in Washington, D.C.

Visit us at booth #417 in the Walter E. Washington Convention Center. Attend our workshops and view our poster presentations. Don’t forget to participate in our booth quiz and win attractive prizes!

Visit the Event Website

Workshops

Saturday, May 13, 2017   |   10:00 am – 10:45 am

New MILLIPLEX® map High Sensitivity Cytokine Panels

Speaker: Robert Keith, R&D

The ability to measure picogram levels of multiple cytokine proteins is critical for understanding the pathogenesis of many diseases. MilliporeSigma introduces three high sensitivity MILLIPLEX® MAP panels to help researchers detect low levels of multiple cytokines in small amounts of sample: the Human High Sensitivity T Cell Magnetic Bead Panel (in both 96-well format and new 384-well format) and a new Mouse High Sensitivity T Cell Magnetic Bead Panel in 96-well format. Both the human and mouse high sensitivity panels can detect picogram levels of cytokines in just 25 µL of sample for up to 21 or 18 critical cytokines, respectively. Representative data will be shown for the use of these panels in the study of inflammation, diabetes (human) and mouse models of aging and metabolic disease.

Sunday, May 14, 2017   |   10:00 am – 10:45 am

Imaging Flow Cytometry: An Embarrassment of Riches

Speakers: Haley Pugsley, Ph.D. Senior Research Scientist, Amnis – part of MilliporeSigma
Margery Pelletier, Ph.D. Candidate, Biomedical and Biomedical Engineering, University of Massachusetts Lowell

2017 ushered in a special edition of Methods with a collection of manuscripts all reporting imaging flow cytometry applications using the Amnis® imaging flow cytometers, FlowSight® and ImageStream®. Applications ranging from the analysis of immune synapses and leukemia to extracellular vesicles and individual chromosomes are reported. Contributions from every corner of the world include Roswell Park Cancer Institute, NIH, Weizmann Institute of Science, University of Western Australia, Health Canada, Cambridge and Moscow State University. Come to our workshop to hear Haley Pugsley’s contribution on studying autophagic flux, Margery Pelletier’s ex vivo culture techniques for hematopoietic stem cells, characterization of netosis and phagocytosis as well as several other applications from the other authors.

Monday, May 15, 2017   |   11:15 am – 12:00 Noon

Automated cell culture for dynamic analysis of immune cell function in real time

Speaker: Amedeo Cappione, PhD, Senior Technology Lead

Precise regulation of lymphocyte function is critical to mounting a specific immune response, and is dependent upon a complex array of cell-cell interactions and responses to an ever-changing microenvironment. Understanding the mechanisms underlying these interactions is essential for development of therapeutics to enhance immune function. Techniques enabling live cell analysis with more in vivo-like conditions provide key insights into dynamic processes. The microfluidics–based CellASIC® ONIX2 System offers precise real-time control of media perfusion, reagent.

Poster Presentations

Saturday, May 13, 2017   |   2:30 pm – 3:45 pm

Dynamic characterization of the phagocytic process utilizing an automated microfluidic platform

Presenter: Cindy Chen

Saturday, May 13, 2017   |   2:30 pm – 3:45 pm

Biological evaluation of the MILLIPLEX® map Mouse High Sensitivity T Cell Panel

Presenter: Bob Keith

Sunday, May 14, 2017   |   2:30 pm – 3:45 pm

A novel method to classify subpopulations in murine spleen and blood using multi-parametric imaging flow cytometry

Presenter: Raymond Kong

Sunday, May 14, 2017   |   2:30 pm – 3:45 pm

Illuminating T cell – APC interactions using multispectral imaging flow cytometry

Presenter: Haley Pugsley