194933 AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem

194933
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      194933-5GM
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          Description
          OverviewGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.3. Mesh: >100.
          Catalogue Number194933
          Brand Family Calbiochem®
          References
          ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
          Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
          Product Information
          CAS number53092-90-3
          ATP CompetitiveN
          FormDark red solid
          ReversibleN
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetGeneral proteolytic substrate
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem MSDS

          Title

          Safety Data Sheet (SDS) 

          AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem Certificates of Analysis

          TitleLot Number
          194933

          References

          Reference overview
          Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
          Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision31-October-2016 JSW
          DescriptionGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 5 mg/ml buffer, 10 min at 37°C, pH 7.0): <0.3. Mesh: >100.
          FormDark red solid
          Recommended reaction conditions
          Assay Protocol for Protease Activity 1. Dissolve 50 mg AZOCOLL® in 5 ml of buffer solution (e.g., PBS, pH 7.0) containing the proteolytic enzyme. 2. Incubate in stoppered tubes for 15 min at 37°C, agitating gently at 5 min intervals. 3. Cool the tubes in ice water and filter the contents through Whatman No. 1 filter paper. 4. Measure the absorbance at 520 nm, subtracting the blank value. 5. Plot absorbance versus protease activity. A linear relationship exists between protease concentration and the rate of dye liberation. Note: The rate of color yield from AZOCOLL® will vary with the individual proteases tested. This protocol is meant to serve as a guideline and may need to be modified for specific applications.
          CAS number53092-90-3
          SolubilityPBS (10 mg/ml)
          Storage +15°C to +30°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
          Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.