114666 Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem

114666
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₆₂H₈₆N₁₂O₁₆ 50-76-0

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114666-1SET
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      114666-5MG
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          Description
          OverviewAnti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
          Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.
          Catalogue Number114666
          Brand Family Calbiochem®
          SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
          References
          ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
          Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
          Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
          Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
          Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
          Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
          White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
          Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
          Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
          Product Information
          CAS number50-76-0
          FormRed crystalline solid
          Hill FormulaC₆₂H₈₆N₁₂O₁₆
          Chemical formulaC₆₂H₈₆N₁₂O₁₆
          Hygroscopic Hygroscopic
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          ApplicationActinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.
          Biological Information
          Primary Targetserine proteases
          Secondary targetcell growth and colony formation in synchronized HeLa cells
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSAU1575000
          Safety Information
          R PhraseR: 28

          Very toxic if swallowed.
          S PhraseS: 28-36/37-45


          Wear suitable protective clothing and gloves.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem MSDS

          Title

          Safety Data Sheet (SDS) 

          Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem Certificates of Analysis

          TitleLot Number
          114666

          References

          Reference overview
          Nakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
          Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
          Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
          Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
          Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
          Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
          White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
          Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
          Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure
          Tools and Tips for Analyzing Apoptosis

          Citations

          Title
        • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
        • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
        • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2009 JSW
          SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
          DescriptionAntineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
          FormRed crystalline solid
          CAS number50-76-0
          RTECSAU1575000
          Chemical formulaC₆₂H₈₆N₁₂O₁₆
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (1 mg/ml), Methanol, or Chloroform
          Storage +2°C to +8°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Merck USA index14, 2800
          ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
          Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
          Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
          Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
          Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
          Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
          White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
          Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
          Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
          Citation
        • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
        • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
        • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.