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MABC1111 Anti-Angiogenin Antibody, clone C527

Anti-Angiogenin, clone C527, Cat. No. MABC1111, is a mouse monoclonal antibody that detects Angiogenin and has been tested for use in ELISA, Immunofluorescence, and Western Blotting.

Anti-Angiogenin Antibody, clone C527 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Ang, Ang1, Rnase5, Rnase5a UniProt Number: P21570
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MABC1111
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1111
      DescriptionAnti-Angiogenin Antibody, clone C527
      Alternate Names
      • Angiogenin-1
      • Ribonuclease 5
      • RNase 5
      Background InformationAngiogenin (UniProt: P21570; also known as EC: 3.3.27.-, Angiogenin-1, Ribonuclease 5, RNase 5) is encoded by the Ang (also known as Ang1, Rnase5, Rnase5a) gene (Gene ID: 11727) in murine species. Angiogenin is a homodimeric protein that belongs to the pancreatic ribonuclease family. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. Angiogenin binds to actin on the surface of endothelial cells and is rapidly endocytosed and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA. Its nuclear localization signal is located in amino acids 55-59. Angiogenin induces vascularization of normal and malignant tissues and its activity is regulated by interaction with RNH1 in vivo. The growth stimulatory functions of Angiogenin are mediated through rRNA transcription. It stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. It cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Under stress, Angiogenin is translocated to SGs and mediates the production of tRNA-derived stress-induced small RNA (tiRNA); which enhance cellular survival by simultaneously suppressing global protein translation, saving anabolic energy, and permitting internal ribosomal entry sequence (IRES)-mediated protein translation of anti-apoptotic genes.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Angiogenin, clone C527, Cat. No. MABC1111, is a mouse monoclonal antibody that detects Angiogenin and has been tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Angiogenin in Immunofluorescence applications (Goncalves, K.A., et. al. (2016). Cell. 166(4):894-906).

      ELISA Analysis: A representative lot detected Angiogenin in ELISA applications (Goncalves, K.A., et. al. (2016). Cell. 166(4):894-906).
      Biological Information
      ImmunogenFull length recombinant murine Angiogenin.
      CloneC527
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone C527 detects Angiogenin in murine cells.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ang
      • Ang1
      • Rnase5
      • Rnase5a
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~16 kDa observed; 16.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant mouse angiogenin.

      Western Blotting Analysis: 4 µg/mL of this antibody detected recombinant mouse angiogenin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1111 04054839415265