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PC742 Anti-Aurora A Rabbit pAb

This Anti-Aurora A Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Aurora A.

Anti-Aurora A Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15

PC742
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      OverviewRecognizes the ~48 kDa Aurora A protein in HEK293 and BT474 cells.

      This product has been discontinued.
      Catalogue NumberPC742
      Brand Family Calbiochem®
      SynonymsAnti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15
      Application Data
      Detection of human aurora A by immunoblotting. Samples: Whole cell lysate from SKBr3 cells (figure A) and immunoprecipitation followed by imunnoblot with AsPC-1 cells (figure B). Primary antibody: Anti-Aurora A Rabbit pAb (Cat. No. PC742) (1:20,000). Detection: chemiluminescence.
      References
      ReferencesCheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
      Chen, S.S., et al. 2002. EMBO J. 21, 4491.
      Zhou, H., et al. 1998. Nat. Genet. 20, 189.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlSKBr3 cells, ASPC-1 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (0.08 µg/ml)
      Immunoprecipitation (5 µl/250 µg total protein)
      Paraffin Sections (1:250-1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids encoded within exon 5 of human Aurora A
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC742 0

      Documentation

      Anti-Aurora A Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC742

      References

      Reference overview
      Cheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
      Chen, S.S., et al. 2002. EMBO J. 21, 4491.
      Zhou, H., et al. 1998. Nat. Genet. 20, 189.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15
      ApplicationImmunoblotting (0.08 µg/ml)
      Immunoprecipitation (5 µl/250 µg total protein)
      Paraffin Sections (1:250-1:1000)
      Application Data
      Detection of human aurora A by immunoblotting. Samples: Whole cell lysate from SKBr3 cells (figure A) and immunoprecipitation followed by imunnoblot with AsPC-1 cells (figure B). Primary antibody: Anti-Aurora A Rabbit pAb (Cat. No. PC742) (1:20,000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~48 kDa Aurora-A protein.
      BackgroundAurora-A is a serine/threonine kinase which has been reported to interact with p53. Overexpression of Aurora-A leads to centrosome amplification, chromosomal instability, and transformation.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids encoded within exon 5 of human Aurora A
      IsotypeIgG
      Specieshuman
      Positive controlSKBr3 cells, ASPC-1 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesCheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
      Chen, S.S., et al. 2002. EMBO J. 21, 4491.
      Zhou, H., et al. 1998. Nat. Genet. 20, 189.