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07-2189 Anti-CAPZIP Antibody

Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.

Anti-CAPZIP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: MK2S4, CAPZIP UniProt Number: Q6JBY9
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07-2189
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2189
      DescriptionAnti-CAPZIP Antibody
      Alternate Names
      • RCSD domain containing 1
      • capZ-interacting protein
      • Protein kinase substrate CapZIP
      • protein kinase substrate MK2S4
      • RCSD domain-containing protein 1
      Background InformationCAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human CAPZIP.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MK2S4
      • CAPZIP
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Stress-induced phosphorylation of CAPZIP may regulate the ability of F-actin-capping protein to remodel actin filament assembly.

      SUBUNIT STRUCTURE: Interacts with CAPZA2 and CAPZB.

      TISSUE SPECIFICITY: Highly expressed in skeletal muscle and more weakly in cardiac muscle. Also expressed in several lymphoid organs, including spleen, thymus, peripheral blood leukocytes, lymph node and bone marrow.

      POST-TRANSLATIONAL MODIFICATION: Dephosphorylation results in its dissociation from CAPZA2.

      SEQUENCE SIMILARITIES: Contains 1 RCSD domain.

      SEQUENCE CAUTION: The sequence CAB70910.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2189 04053252629457