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MAB3678-I-100UL Anti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1

This mouse monoclonal Anti-Chloramphenicol Acetyltransferase, clone CAT-1, Cat. No. MAB3678-I is tested for use in Immunocytochemistry and Western Blotting and Immunofluorescence, for the detection of Chloramphenicol acetyltransferase.

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MAB3678-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB3678-I-100UL
      DescriptionAnti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1
      Alternate Names
      • EC:2.3.1.28
      • CAT
      Background InformationChloramphenicol acetyltransferase (UniProt: P62577; also known as EC:2.3.1.28, CAT) is encoded by the Cat gene (Gene ID: 59683728) in E. coli. Chloramphenicol acetyltransferase is an enzyme that catalyzes the acetyl-S-CoA-dependent acetylation of chloramphenicol at the 3-hydroxyl group leading to the production of 3-acetoxy chloramphenicol that does not bind to bacterial ribosomes and does not inhibit peptidyltransferase activity. It displays high stability in cells and has a half-life of about 48 hours. A highly reactive histidine residue has been implicated in the catalytic mechanism. CAT is shown to be an effector of chloramphenicol resistance in bacteria. Its synthesis is shown to be constitutive in E. coli and other Gram-negative bacteria that harbor plasmids bearing the structural gene for the enzyme. However, in Gram-positive bacteria such as staphylococci and streptococci it is synthesized only in the presence of chloramphenicol and related compounds. (Ref.:Shaw WH. (1983). CRC Crit. Rev. Biochem. 14(1); 1-46).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Chloramphenicol Acetyltransferase, clone CAT-1, Cat. No. MAB3678-I is tested for use in Immunocytochemistry and Western Blotting and Immunofluorescence, for the detection of Chloramphenicol acetyltransferase.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected Chloramphenicol acetyltransferase in Immunofluorescence applications (Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

      Western Blotting Analysis: A representative lot detected Chloramphenicol acetyltransferase in Western Blotting applications (Smith, M.C., et al. (2011). Antiviral Res.;91(3):259-66; Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant chloramphenicol acetyl transferase.
      EpitopeUnknown
      CloneCAT-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CAT-1 is a mouse monoclonal antibody that detects Chloramphenicol acetyltransferase.
      IsotypeIgG1
      Species Reactivity
      • E. coli
      Species Reactivity NoteE. Coli. Predicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cat
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight25.66 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HEK293 cells transfected with gWIZ CAT plasmid.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Chloramphenicol Acetyltransferase in HEK293 cells transfected with gWIZ CAT plasmid.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3678-I-100UL 04065266791433