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PC127 Anti-DNA-PK (Ab-1) (2015-2134) Rabbit pAb

This Anti-DNA-PK (Ab-1) (2015-2134) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of DNA-PK (Ab-1) (2015-2134).

Anti-DNA-PK (Ab-1) (2015-2134) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-DNA Dependent Protein Kinase

PC127
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Eq, H, M, R, XnRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~400-470 kDa DNA-PK protein in rodent cell lines.
      Catalogue NumberPC127
      Brand Family Calbiochem®
      SynonymsAnti-DNA Dependent Protein Kinase
      References
      ReferencesConnelly, M.A., et al. 1997. Genomics, Submitted.
      Kharbanda, S., et al. 1997. Nature 386, 732.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
      Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
      Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
      Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
      Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
      Connelly, M.A., et al. 1996. Gene 175, 271.
      Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
      Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
      Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
      Song, Q., et al. 1996. EMBO J. 15, 3238.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Blunt, T., et al. 1995. Cell 80, 813.
      Hartley, K.O., et al. 1995. Cell 82, 849.
      Kirchgessner, C.U., et al. 1995. Science 267 1178.
      Lees-Miller, S.P., et al. 1995. Science 267, 1183.
      Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
      Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
      Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131.
      Product Information
      FormLiquid
      FormulationIn 0.01 M sodium phosphate buffer, 1% BSA.
      Negative controlMO59J cells
      Positive controlMO59K cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunofluorescence and Paraffin Sections Moll, U. et al. 1999. Oncogene 18, 3114.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunofluorescence (see application references)
      Immunoprecipitation (0.5 µg)
      Paraffin Sections (see application references)
      Application CommentsReactivity towards DNA-PK from other species may also occur. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant fusion protein consisting of amino acids 2015-2134 of human DNA-PK catalytic subunit
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Horse
      • Human
      • Mouse
      • Rat
      • Xenopus
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC127 0

      Documentation

      Anti-DNA-PK (Ab-1) (2015-2134) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DNA-PK (Ab-1) (2015-2134) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC127

      References

      Reference overview
      Connelly, M.A., et al. 1997. Genomics, Submitted.
      Kharbanda, S., et al. 1997. Nature 386, 732.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
      Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
      Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
      Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
      Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
      Connelly, M.A., et al. 1996. Gene 175, 271.
      Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
      Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
      Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
      Song, Q., et al. 1996. EMBO J. 15, 3238.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Blunt, T., et al. 1995. Cell 80, 813.
      Hartley, K.O., et al. 1995. Cell 82, 849.
      Kirchgessner, C.U., et al. 1995. Science 267 1178.
      Lees-Miller, S.P., et al. 1995. Science 267, 1183.
      Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
      Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
      Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-October-2007 RFH
      SynonymsAnti-DNA Dependent Protein Kinase
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunofluorescence (see application references)
      Immunoprecipitation (0.5 µg)
      Paraffin Sections (see application references)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~400-470 kDa DNA-PK catalytic subunit.
      BackgroundDNA Dependent Protein Kinase, DNA-PK, is a nuclear serine/threonine protein kinase composed of two subunits, a ~470 kDa catalytic subunit, DNA-PKcs, and the Ku autoantigen, the heterodimeric DNA binding protein p70/p80. DNA-PK has been shown to phosphorylate a number of key proteins in vitro including p53, Sp1, myc, RPA, and RNA Pol I. DNA-PK activity requires association of DNA-PKcs with Ku, DNA binding, and the presence of DNA ends. Mutations in DNA-PKcs contribute to murine SCID phenotypes which leads to inefficient V(D)J recombination in developing T and B lymphocytes. Loss of DNA-PK activity in fibroblasts or lymphocytes also confers on cells hypersensitivity to killing by ionizing radiation. These two phenotypes implicate DNA-PK in the repair of DNA double strand breaks in all cells and V(D)J recombination in lymphocytes. The 4127 amino acid DNA-PKcs protein shares a high degree of similarity with the ATM family of kinases, including ATM, ATR, and FRAP in humans and MEC1 and TEL1 in yeast. The catalytic domain of DNA PKcs also shares homology with the PI3K family of lipid kinases and DNA-PK is inhibitable by wortmanin, a lipid kinase inhibitor. While the exact role for DNA-PK in cell cycle regulation is unclear, the protein may be important for suppression of apoptosis since DNA-PKcs is targeted for proteolysis during apoptosis, which leads to loss of catalytic activity. DNA-PK can also be inactivated through phosphorylation, possibly by c-abl and by autophosphorylation. DNA-PKcs is expressed in all cells and a DNA-PK activity is observed in all higher eukaryotes examined. The protein level of DNA-PKcs in human cells is very high, 50 to 100x that observed in murine cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant fusion protein consisting of amino acids 2015-2134 of human DNA-PK catalytic subunit
      IsotypeIgG
      SpeciesXenopus, horse, human, mouse, rat
      Positive controlMO59K cells
      Negative controlMO59J cells
      FormLiquid
      FormulationIn 0.01 M sodium phosphate buffer, 1% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsReactivity towards DNA-PK from other species may also occur. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesConnelly, M.A., et al. 1997. Genomics, Submitted.
      Kharbanda, S., et al. 1997. Nature 386, 732.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Peterson, S.R., et al. 1997. J. Biol. Chem. 272, 10227.
      Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol. 217, 91.
      Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10285.
      Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol. 8, 153.
      Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem. 271, 8936.
      Connelly, M.A., et al. 1996. Gene 175, 271.
      Danska, J.S., et al. 1996. Mol. Cell. Biol. 16, 5507.
      Han, Z., et al. 1996. J. Biol. Chem. 271, 25035.
      Lees-Miller, S.P. 1996. Biochem. Cell. Biol. 74, 503.
      Song, Q., et al. 1996. EMBO J. 15, 3238.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Blunt, T., et al. 1995. Cell 80, 813.
      Hartley, K.O., et al. 1995. Cell 82, 849.
      Kirchgessner, C.U., et al. 1995. Science 267 1178.
      Lees-Miller, S.P., et al. 1995. Science 267, 1183.
      Poltoratsky, V.P., et al. 1995. J. Immunol. 155, 4529.
      Anderson, C.W. 1993. Trends Biochem. Sci. 18, 433.
      Gottleib, T.M. and Jackson, S.P. 1993. Cell 72, 131.
      Application referencesImmunofluorescence and Paraffin Sections Moll, U. et al. 1999. Oncogene 18, 3114.