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GR01L Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Anti-EGFR (Ab-1) Mouse mAb (528) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epidermal Growth Factor Receptor

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GR01L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the EGF receptor protein in A431 cells and normal skin.
      Catalogue NumberGR01L
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor
      References
      ReferencesHunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
      Positive controlA-431 cells or skin tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (see application references)
      Application CommentsThis antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from human A431 cells
      ImmunogenHuman
      Clone528
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR01L 0

      Documentation

      Anti-EGFR (Ab-1) Mouse mAb (528) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EGFR (Ab-1) Mouse mAb (528) Certificates of Analysis

      TitleLot Number
      GR01L

      References

      Reference overview
      Hunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 JSW
      SynonymsAnti-Epidermal Growth Factor Receptor
      ApplicationFlow Cytometry (5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
      BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human EGFR from human A431 cells
      Clone528
      IsotypeIgG2a
      Specieshuman, not mouse, not rat
      Positive controlA-431 cells or skin tissue
      FormLyophilized
      FormulationLyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
      PreservativeNone
      CommentsThis antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesHunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Application referencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies