Millipore Sigma Vibrant Logo

MABS1915 Anti-EGFRvIII Antibody, clone DH8.3

Anti-EGFRvIII, clone DH8.3, Cat. No. MABS1915, is a mouse monoclonal antibody that specifically detects epidermal growth factor receptor vIIIa and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-EGFRvIII Antibody, clone DH8.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: EGFR, ERBB, ERBB1, HER1 UniProt Number: P00533
View Products on Sigmaaldrich.com
MABS1915
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1915
      DescriptionAnti-EGFRvIII Antibody, clone DH8.3
      Alternate Names
      • Proto-oncogene c-ErbB-1
      • Receptor tyrosine-protein kinase erbB-1
      • Epidermal growth facttor receptor vIII
      Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved. It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain. EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC). It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance. Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth. (Ref.: Ji, H., et al. (2006). Proc. Natl. Acad. Sci. USA 103(20); 7817-7822).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-EGFRvIII, clone DH8.3, Cat. No. MABS1915, is a mouse monoclonal antibody that specifically detects epidermal growth factor receptor vIIIa and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected EGFRvIII in Western Blotting applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

      Flow Cytometry Analysis: A representative lot detected EGFRvIII in Flow Cytometry applications (Johns, T.G., et. al. (2002). Int J Cancer. 98(3):398-408; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

      Immunohistochemistry Analysis: A representative lot detected EGFRvIII in Immunohistochemistry applications (Jungbluth, A.A., et. al. (2003). Proc Natl Acad Sci USA. 100(2):639-44; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Feng, H., et. al. (2014). J Clin Invest. 124(9):3741-56; Feng, H., et. al. (2012). Proc Natl Acad Sci USA. 109(8):3018-23.

      Immunoprecipitation Analysis: A representative lot detected EGFRvIII in Immunoprecipitation applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

      ELISA Analysis: A representative lot detected EGFRvIII in ELISA applications (Johns, T.G., et. al. (2002). Int J Cancer. 98(3):398-408; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to EGFRvIII (EGFR variant III) (LEEKKGNYVVTDHC) sequence.
      CloneDH8.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone DH8.3 detects EGFR variant vIII a truncated form of EGFR, present in human cancer cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB
      • ERBB1
      • HER1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed; 134.28 kDa calculalted. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U87MG.∆EGFR cell lysates.

      Western Blotting Analysis: 4 ug/mL of this antibody detected EGFRvIII in 10 µg of U87MG.∆EGFR cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1915 04054839321771

      Documentation

      Anti-EGFRvIII Antibody, clone DH8.3 Certificates of Analysis

      TitleLot Number
      Anti-EGFRvIII, clone DH8.3 - 3120104 3120104
      Anti-EGFRvIII, clone DH8.3 - 3429043 3429043
      Anti-EGFRvIII, clone DH8.3 - 3879547 3879547
      Anti-EGFRvIII, clone DH8.3 - 4097741 4097741
      Anti-EGFRvIII, clone DH8.3 - 4215405 4215405
      Anti-EGFRvIII, clone DH8.3 - 4252138 4252138
      Anti-EGFRvIII, clone DH8.3 - 4295868 4295868
      Anti-EGFRvIII, clone DH8.3 Monoclonal Antibody 2990467
      Anti-EGFRvIII, clone DH8.3 Monoclonal Antibody Q2898873
      Anti-EGFRvIII, clone DH8.3 Monoclonal Antibody 3079576
      Show more