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06-935 Anti-Estrogen Receptor α Antibody

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06-935
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P), IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-935
      DescriptionAnti-Estrogen Receptor α Antibody
      Alternate Names
      • ER-alpha
      • Estradiol receptor
      • Nuclear receptor subfamily 3 group A member 1
      Background InformationEstrogen receptor (UniProt: P06211; also known as ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1) is encoded by the Esr1 (also known as Esr, Estr, Nr3a1) gene (Gene ID: 24890) in rat. Estrogen receptor a (ERa) is a steroid hormone receptor that is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. Its expression has been reported in mammary gland, uterus, ovary, bone, prostrate, testes, and adipose tissue. In the absence of ligand, it is weakly associated with nuclear components and upon hormone binding its affinity is greatly enhanced. Its DNA binding region is localized to amino acids 190-255 (Domain C), which is highly conserved and contains two Cys2Cys2 zinc finger motifs. This region is essential and sufficient for binding of ER to the estrogen response element (ERE). Domain E, the ligand-binding domain (LBD; aa 316-552), is also highly conserved and is responsible for interaction of the receptor with various estrogens and antiestrogens. In ERa, transcription activation function-1 (AF-1) is localized in the amino-terminal A/B domain (aa 1-189) of the receptor and serves as a ligand-independent transcription activation domain. Activation function-2 (AF-2) resides in the hormone-binding domain (aa 316-600) and acts as a ligand-inducible activation domain. ERa can undergo phosphorylation by CDK2/cyclin A and by CK1 that enhances its transcriptional activity. In many breast cancers, ER activation by estrogens is shown to be responsible for enhanced proliferation, which is counteracted by the presence of ERβ that exerts an antiproliferative effect. (Ref. Paterni, I., et al. (2014). Steroids. 90; 13-29).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • MCF7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesTested applications

      Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Estrogen Receptor in human stomach, human uterine cancer, human breast cancer and human cervical cancer tissue sections.

      Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen Receptor in MCF-7 cell lysate.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Estrogen Receptor in MCF-7 cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of rat Estrogen Receptor alpha.
      EpitopeC-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects estrogen receptor a. It targets an epitope within 15 amino acids from the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryActs as a transcriptional activator when bound to estrogen; may play a role in myocardial regulation.
      Gene Symbol
      • Esr1
      • Esr
      • Estr
      • Nr3a1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

      SUBUNIT STRUCTURE: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with the NCOA3, NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is ligand-inducible. Interacts with AKAP13, CUEDC2, HEXIM1, KDM5A, PELP1, PHB2, SLC30A9 and SMARD1. Interacts with MUC1; the interaction is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with MLL2. Interacts with ATAD2; the interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via its C-terminus). Interacts with MACROD1. Interacts with FOXL2. Interacts with SH2D4A and PLCG. Interacts with SH2D4A; the interaction blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62; the interaction requires estradiol and appears to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with PRMT2. Interacts with PIK3R1 or PIK3R2, SRC and PTK2. Interacts with RBFOX2. Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with DYX1C1, MAP1S and UBE1C.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
      Molecular Weight~58 kDa observed; 67.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      06-935 04053252510755

      Documentation

      Anti-Estrogen Receptor α Antibody MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Estrogen Receptor α Antibody Certificates of Analysis

      TitleLot Number
      Anti-Estrogen Receptor (C1355) - 1988421 1988421
      Anti-Estrogen Receptor (C1355) - 2197507 2197507
      Anti-Estrogen Receptor (C1355) - 2272072 2272072
      Anti-Estrogen Receptor (C1355) - 2318775 2318775
      Anti-Estrogen Receptor (C1355) - DAM1682137 DAM1682137
      Anti-Estrogen Receptor (C1355) - DAM1791348 DAM1791348
      Anti-Estrogen Receptor (C1355) - NG1838275 NG1838275
      Anti-Estrogen Receptor #225; (C1355) - DAM1591762 DAM1591762
      Anti-Estrogen Receptor #945; (C1355) - 2120112 2120112
      Anti-Estrogen Receptor #945; (C1355) - 2147326 2147326

      References

      Reference overviewApplicationSpeciesPub Med ID
      Characterization of aromatase expression in the adult male and female mouse brain. I. Coexistence with oestrogen receptors α and β, and androgen receptors.
      Stanić, D; Dubois, S; Chua, HK; Tonge, B; Rinehart, N; Horne, MK; Boon, WC
      PloS one  9  e90451  2014

      Show Abstract
      24646567 24646567
      Estrogens stimulate serotonin neurons to inhibit binge-like eating in mice.
      Cao, X; Xu, P; Oyola, MG; Xia, Y; Yan, X; Saito, K; Zou, F; Wang, C; Yang, Y; Hinton, A; Yan, C; Ding, H; Zhu, L; Yu, L; Yang, B; Feng, Y; Clegg, DJ; Khan, S; DiMarchi, R; Mani, SK; Tong, Q; Xu, Y
      The Journal of clinical investigation  124  4351-62  2014

      Show Abstract
      25157819 25157819
      Social isolation during puberty affects female sexual behavior in mice.
      Kercmar, J; Tobet, SA; Majdic, G
      Frontiers in behavioral neuroscience  8  337  2014

      Show Abstract
      Immunohistochemistry25324747 25324747
      Neurochemical characterization and sexual dimorphism of projections from the brain to abdominal and subcutaneous white adipose tissue in the rat.
      Adler, ES; Hollis, JH; Clarke, IJ; Grattan, DR; Oldfield, BJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  15913-21  2012

      Show Abstract
      Immunohistochemistry23136429 23136429
      Estradiol treatment prevents injury induced enhancement in spinal cord dynorphin expression.
      Gupta, DS; Hubscher, CH
      Frontiers in physiology  3  28  2012

      Show Abstract
      ImmunohistochemistryRat22371702 22371702
      The impact of neonatal bisphenol-A exposure on sexually dimorphic hypothalamic nuclei in the female rat.
      Adewale, HB; Todd, KL; Mickens, JA; Patisaul, HB
      Neurotoxicology  32  38-49  2011

      Show Abstract
      Immunohistochemistry20696184 20696184
      Radiation acts on the microenvironment to affect breast carcinogenesis by distinct mechanisms that decrease cancer latency and affect tumor type.
      Nguyen, DH; Oketch-Rabah, HA; Illa-Bochaca, I; Geyer, FC; Reis-Filho, JS; Mao, JH; Ravani, SA; Zavadil, J; Borowsky, AD; Jerry, DJ; Dunphy, KA; Seo, JH; Haslam, S; Medina, D; Barcellos-Hoff, MH
      Cancer cell  19  640-51  2011

      Show Abstract
      Immunohistochemistry21575864 21575864
      Roles for gamma-aminobutyric acid in the development of the paraventricular nucleus of the hypothalamus.
      Kristy M McClellan,Matthew S Stratton,Stuart A Tobet
      The Journal of comparative neurology  518  2010

      Show Abstract Full Text Article
      20506472 20506472
      Expression of aromatase, androgen and estrogen receptors in peripheral target tissues in diabetes.
      Prabhu, A; Xu, Q; Manigrasso, MB; Biswas, M; Flynn, E; Iliescu, R; Lephart, ED; Maric, C
      Steroids  75  779-87  2010

      Show Abstract Full Text Article
      20064538 20064538
      Role of Estradiol in the Dynamic Control of Tanycyte Plasticity Mediated by Vascular Endothelial Cells in the Median Eminence.
      de Seranno S, d'Anglemont de Tassigny X, Estrella C, Loyens A, Kasparov S, Leroy D, Ojeda SR, Beauvillain JC, Prevot V
      Endocrinology  151  1760-72 Epub 2010 Feb 4  2010

      Show Abstract Full Text Article
      20133455 20133455