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MABE960 Anti-Fox1 Antibody, clone A2BP1

Anti-Fox1, clone A2BP1, Cat. No. MABE960, is a highly specific rat monoclonal antibody that targets Fox1 and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.

Anti-Fox1 Antibody, clone A2BP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: RBFOX1, A2BP, A2BP1, FOX1, HRNBP1 UniProt Number: Q9NWB1
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MABE960
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, IH(P), IF, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE960
      DescriptionAnti-Fox1 Antibody, clone A2BP1
      Alternate Names
      • RNA binding protein fox-1 homolog 1
      • Ataxin-2-binding protein 1
      • Fox-1 homolog A
      • Hexaribonucleotide-binding protein 1
      Background InformationRNA binding protein fox-1 homolog 1 (UniProt Q9NWB1; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide-binding protein 1) is encoded by the RBFOX1 (also known as A2BP, A2BP1, FOX1, HRNBP1) gene (Gene ID 54715) in human. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization, resulting in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leading to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.
      References
      Product Information
      FormatPurified
      PresentationPurified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fox1, clone A2BP1, Cat. No. MABE960, is a highly specific rat monoclonal antibody that targets Fox1 and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Fox1 in mouse cerebral cortex and heart tissue sections.

      Immunocytochemistry Analysis: A representative lot immunostained exclusively differentiated neurons, but not neural stem cells, neuronal progenitors, astrocytes, or oligodendrocytes derived from p53-/-Pten+/- murine premalignant neural/progenitor stem cells (M-NSCs) cultured under various differentiation conditions (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

      Immunofluorescence Analysis: A representative lot immunostained postmitotic terminally differentiated neurons in brain sections from 2-week old postnatal mice (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).

      Immunohistochemistry Analysis: A representative lot detected downregulated Fox1 immunoreactivity in more than 90% of human GBM tissue samples when compared with normal human brain tissues (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).
      Biological Information
      ImmunogenRecombinant full-length human Fox1.
      CloneA2BP1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityThis monoclonal antibody immunostained exclusively differentiated neurons, but not neural stem cells, neuronal progenitors, astrocytes, or oligodendrocytes derived from p53-/-Pten+/- murine premalignant neural/progenitor stem cell (M-NSC) cultures (Hu, J., et al. (2013). Proc. Natl. Acad. Sci. U.S.A. 110(36):14520-14527).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RBFOX1
      • A2BP
      • A2BP1
      • FOX1
      • HRNBP1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight42.78/44.79/40.01/42.08/42.40 kDa (human isoform 1/2 (gamma)/3/4 (alpha)/5 (beta)) and 42.68/40.49/44.73/40.71/40.29/42.34/38.92 kDa (mouse isoform 1 (A016)/2/3 (A713)/4/5 (A030)/6 (A715)/7 (A704)) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebellum tissue section.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Fox1 in human cerebellum tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE960 04054839112331